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==CdiA-CT from uropathogenic Escherichia coli in complex with cognate immunity protein and CysK==
==CdiA-CT from uropathogenic Escherichia coli in complex with cognate immunity protein and CysK==
<StructureSection load='5j5v' size='340' side='right' caption='[[5j5v]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
<StructureSection load='5j5v' size='340' side='right'caption='[[5j5v]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5j5v]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5J5V FirstGlance]. <br>
<table><tr><td colspan='2'>[[5j5v]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_536 Escherichia coli 536] and [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J5V FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=IT1:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>IT1</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5j43|5j43]], [[5j4a|5j4a]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cysteine_synthase Cysteine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.47 2.5.1.47] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j5v OCA], [https://pdbe.org/5j5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j5v RCSB], [https://www.ebi.ac.uk/pdbsum/5j5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j5v ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5j5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j5v OCA], [http://pdbe.org/5j5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j5v RCSB], [http://www.ebi.ac.uk/pdbsum/5j5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j5v ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/CDII_ECOL5 CDII_ECOL5]] Immunity protein component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. CDI modules allow bacteria to communicate with and inhibit the growth of closely related neighboring bacteria in a contact-dependent fashion. Protects cells against the tRNA nuclease activity of CdiA, its cognate toxin protein, but not against CdiA from E.coli strain EC93 or D.dadantii strain 3937.<ref>PMID:21085179</ref> <ref>PMID:22333533</ref> <ref>PMID:24889811</ref>  [[http://www.uniprot.org/uniprot/CDIA_ECOL5 CDIA_ECOL5]] Toxic component of a toxin-immunity protein module, which functions as a cellular contact-dependent growth inhibition (CDI) system. CDI modules allow bacteria to communicate with and inhibit the growth of closely related neighboring bacteria in a contact-dependent fashion (target cell counts decrease 100- to 1000-fold). CdiA toxicity is neutralized by its cognate immunity protein CdiI, but not by CdiI from other bacteria (PubMed:23469034, PubMed:24889811). BamA on the target cells acts as a receptor for intact CdiA (PubMed:23469034). However isolated CdiA-CT is imported in an F-pilus-mediated fashion; CdiA-CT inhibits F-mediated conjugation, probably via its N-terminus (residues 3016-3097), although it is not clear if this is physiologically significant (PubMed:24889811). The C-terminal domain (CT) cleaves within tRNA anticodon loops (PubMed:22333533, PubMed:24889811); this activity is inhibited by cognate CdiI (PubMed:21085179, PubMed:22333533, PubMed:24889811). tRNase activity of CdiA-CT is stimulated by CysK, although the extreme C-terminus (residues 3098-3242) has tRNase activity in the absence of CysK. In vivo CDI toxicity requires CysK (PubMed:22333533, PubMed:24889811). Purified CdiA-CT (residues 3016-3242) inhibits E.coli cell growth when added to cultures alone or in complex with cognate CdiI, growth is inhibited when cognate CdiI is present within the cell but not when a CdiA-CT/CdiI complex is added extracellularly, suggesting CdiA-CT alone but not the CdiA-CT/CdiI complex is imported into the target cell (PubMed:24889811).<ref>PMID:21085179</ref> <ref>PMID:22333533</ref> <ref>PMID:23469034</ref> <ref>PMID:24889811</ref> <ref>PMID:25174572</ref> 
[https://www.uniprot.org/uniprot/A0A454A691_ECOL5 A0A454A691_ECOL5]  
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Cysteine synthase]]
[[Category: Escherichia coli 536]]
[[Category: Goulding, C W]]
[[Category: Escherichia coli O157:H7]]
[[Category: Johnson, P M]]
[[Category: Large Structures]]
[[Category: Morse, R P]]
[[Category: Goulding CW]]
[[Category: Complex]]
[[Category: Johnson PM]]
[[Category: Endonuclease]]
[[Category: Morse RP]]
[[Category: Immunity protein]]
[[Category: Toxin]]
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