5l2b: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Structure of CNTnw N149S, E332A in an outward-facing state== | ==Structure of CNTnw N149S, E332A in an outward-facing state== | ||
<StructureSection load='5l2b' size='340' side='right' caption='[[5l2b]], [[Resolution|resolution]] 3.80Å' scene=''> | <StructureSection load='5l2b' size='340' side='right'caption='[[5l2b]], [[Resolution|resolution]] 3.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5l2b]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L2B OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[5l2b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_wadsworthii_9715 Neisseria wadsworthii 9715]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5L2B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5L2B FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6ZL:2-{[(4-O-ALPHA-D-GLUCOPYRANOSYL-BETA-D-GLUCOPYRANOSYL)OXY]METHYL}-2-OCTYLDECYL+4-O-ALPHA-D-GLUCOPYRANOSYL-BETA-D-GLUCOPYRANOSIDE'>6ZL</scene> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.8Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6ZL:2-{[(4-O-ALPHA-D-GLUCOPYRANOSYL-BETA-D-GLUCOPYRANOSYL)OXY]METHYL}-2-OCTYLDECYL+4-O-ALPHA-D-GLUCOPYRANOSYL-BETA-D-GLUCOPYRANOSIDE'>6ZL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5l2b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5l2b OCA], [https://pdbe.org/5l2b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5l2b RCSB], [https://www.ebi.ac.uk/pdbsum/5l2b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5l2b ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/G4CRQ5_9NEIS G4CRQ5_9NEIS] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Membrane transporters move substrates across the membrane by alternating access of their binding sites between the opposite sides of the membrane. An emerging model of this process is the elevator mechanism, in which a substrate-binding transport domain moves a large distance across the membrane. This mechanism has been characterized by a transition between two states, but the conformational path that leads to the transition is not yet known, largely because the available structural information has been limited to the two end states. Here we present crystal structures of the inward-facing, intermediate, and outward-facing states of a concentrative nucleoside transporter from Neisseria wadsworthii. Notably, we determined the structures of multiple intermediate conformations, in which the transport domain is captured halfway through its elevator motion. Our structures present a trajectory of the conformational transition in the elevator model, revealing multiple intermediate steps and state-dependent conformational changes within the transport domain that are associated with the elevator-like motion. | |||
Visualizing multistep elevator-like transitions of a nucleoside transporter.,Hirschi M, Johnson ZL, Lee SY Nature. 2017 Apr 17. doi: 10.1038/nature22057. PMID:28424521<ref>PMID:28424521</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5l2b" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Neisseria wadsworthii 9715]] | ||
[[Category: | [[Category: Hirschi M]] | ||
[[Category: | [[Category: Johnson ZL]] | ||
[[Category: | [[Category: Lee S-Y]] | ||
Latest revision as of 12:47, 4 October 2023
Structure of CNTnw N149S, E332A in an outward-facing state
| ||||||||||||
