1ugl: Difference between revisions
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==Solution structure of S8-SP11== | |||
<StructureSection load='1ugl' size='340' side='right'caption='[[1ugl]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1ugl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brassica_rapa Brassica rapa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UGL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UGL FirstGlance]. <br> | |||
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 30 models</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ugl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ugl OCA], [https://pdbe.org/1ugl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ugl RCSB], [https://www.ebi.ac.uk/pdbsum/1ugl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ugl ProSAT], [https://www.topsan.org/Proteins/RSGI/1ugl TOPSAN]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9SE17_BRACM Q9SE17_BRACM] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
== | |||
Many flowering plants possess a self-incompatibility system to prevent inbreeding. In Brassica rapa, self/non-self recognition in mating is established through S-haplotype-specific interactions between stigma receptors and S-locus protein 11 (SP11, also called S-locus cysteine-rich protein) that is encoded at the highly polymorphic S-locus. Here we describe the solution structure of the SP11 protein of the S8-haplotype (S8-SP11), which specifically binds to the stigma factor of the same haplotype. It folds into an alpha/beta sandwich structure that resembles those of plant defensins. Residues important for structural integrity are highly conserved among the allelic SP11s, suggesting the existence of a common folding pattern. Structure-based sequence alignment and homology modeling of allelic SP11 identified a hyper-variable (HV) region, which is thought to form a loop that bulges out from the body of the protein that is amenable to solvent exposure. We suggest that the HV region could serve as a specific binding site for the stigma receptor. | Many flowering plants possess a self-incompatibility system to prevent inbreeding. In Brassica rapa, self/non-self recognition in mating is established through S-haplotype-specific interactions between stigma receptors and S-locus protein 11 (SP11, also called S-locus cysteine-rich protein) that is encoded at the highly polymorphic S-locus. Here we describe the solution structure of the SP11 protein of the S8-haplotype (S8-SP11), which specifically binds to the stigma factor of the same haplotype. It folds into an alpha/beta sandwich structure that resembles those of plant defensins. Residues important for structural integrity are highly conserved among the allelic SP11s, suggesting the existence of a common folding pattern. Structure-based sequence alignment and homology modeling of allelic SP11 identified a hyper-variable (HV) region, which is thought to form a loop that bulges out from the body of the protein that is amenable to solvent exposure. We suggest that the HV region could serve as a specific binding site for the stigma receptor. | ||
Structure of the male determinant factor for Brassica self-incompatibility.,Mishima M, Takayama S, Sasaki K, Jee JG, Kojima C, Isogai A, Shirakawa M J Biol Chem. 2003 Sep 19;278(38):36389-95. Epub 2003 Jun 30. PMID:12835321<ref>PMID:12835321</ref> | |||
Structure of the male determinant factor for Brassica self-incompatibility., Mishima M, Takayama S, Sasaki K, Jee JG, Kojima C, Isogai A, Shirakawa M | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1ugl" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Brassica rapa]] | |||
[[Category: Large Structures]] | |||
[[Category: Isogai A]] | |||
[[Category: Jee JG]] | |||
[[Category: Kojima C]] | |||
[[Category: Mishima M]] | |||
[[Category: Sasaki K]] | |||
[[Category: Shirakawa M]] | |||
[[Category: Takayama S]] | |||
