5vh1: Difference between revisions
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==Crystal Structure of Chicken Gamma S Crystallin== | |||
<StructureSection load='5vh1' size='340' side='right'caption='[[5vh1]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5vh1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5v14 5v14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VH1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VH1 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vh1 OCA], [https://pdbe.org/5vh1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vh1 RCSB], [https://www.ebi.ac.uk/pdbsum/5vh1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vh1 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/D0UYB8_CHICK D0UYB8_CHICK] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Previous attempts to crystallize mammalian gammaS-crystallin were unsuccessful. Native L16 chicken gammaS crystallized avidly while the Q16 mutant did not. The X-ray structure for chicken gammaS at 2.3 A resolution shows the canonical structure of the superfamily plus a well-ordered N arm aligned with a beta sheet of a neighboring N domain. L16 is also in a lattice contact, partially shielded from solvent. Unexpectedly, the major lattice contact matches a conserved interface (QR) in the multimeric beta-crystallins. QR shows little conservation of residue contacts, except for one between symmetry-related tyrosines, but molecular dipoles for the proteins with QR show striking similarities while other gamma-crystallins differ. In gammaS, QR has few hydrophobic contacts and features a thin layer of tightly bound water. The free energy of QR is slightly repulsive and analytical ultracentrifugation confirms no dimerization in solution. The lattice contacts suggest how gamma-crystallins allow close packing without aggregation in the crowded environment of the lens. | |||
Crystal Structure of Chicken gammaS-Crystallin Reveals Lattice Contacts with Implications for Function in the Lens and the Evolution of the betagamma-Crystallins.,Sagar V, Chaturvedi SK, Schuck P, Wistow G Structure. 2017 Jul 5;25(7):1068-1078.e2. doi: 10.1016/j.str.2017.05.015. Epub, 2017 Jun 22. PMID:28648607<ref>PMID:28648607</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Sagar | <div class="pdbe-citations 5vh1" style="background-color:#fffaf0;"></div> | ||
[[Category: Wistow | |||
==See Also== | |||
*[[Crystallin 3D structures|Crystallin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Gallus gallus]] | |||
[[Category: Large Structures]] | |||
[[Category: Sagar V]] | |||
[[Category: Wistow G]] | |||
Latest revision as of 13:48, 4 October 2023
Crystal Structure of Chicken Gamma S Crystallin
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