1urn: Difference between revisions

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[[Image:1urn.jpg|left|200px]]


{{Structure
==U1A MUTANT/RNA COMPLEX + GLYCEROL==
|PDB= 1urn |SIZE=350|CAPTION= <scene name='initialview01'>1urn</scene>, resolution 1.920&Aring;
<StructureSection load='1urn' size='340' side='right'caption='[[1urn]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
<table><tr><td colspan='2'>[[1urn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1URN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1URN FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.92&#8491;</td></tr>
|GENE=
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1urn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1urn OCA], [https://pdbe.org/1urn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1urn RCSB], [https://www.ebi.ac.uk/pdbsum/1urn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1urn ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1urn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1urn OCA], [http://www.ebi.ac.uk/pdbsum/1urn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1urn RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/SNRPA_HUMAN SNRPA_HUMAN] Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. Binds preferentially to the 5'-UGCAC-3' motif in vitro.<ref>PMID:9848648</ref>
 
== Evolutionary Conservation ==
'''U1A MUTANT/RNA COMPLEX + GLYCEROL'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ur/1urn_consurf.spt"</scriptWhenChecked>
The crystal structure of the RNA-binding domain of the small nuclear ribonucleoprotein U1A bound to a 21-nucleotide RNA hairpin has been determined at 1.92 A resolution. The ten-nucleotide RNA loop binds to the surface of the beta-sheet as an open structure, and the AUUGCAC sequence of the loop interacts extensively with the conserved RNP1 and RNP2 motifs and the C-terminal extension of the RNP domain. These interactions include stacking of RNA bases with aromatic side chains of proteins and many direct and water-mediated hydrogen bonds. The structure reveals the stereochemical basis for sequence-specific RNA recognition by the RNP domain.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1URN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1URN OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1urn ConSurf].
 
<div style="clear:both"></div>
==Reference==
== References ==
Crystal structure at 1.92 A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin., Oubridge C, Ito N, Evans PR, Teo CH, Nagai K, Nature. 1994 Dec 1;372(6505):432-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7984237 7984237]
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Evans, P R.]]
[[Category: Evans PR]]
[[Category: Ito, N.]]
[[Category: Ito N]]
[[Category: Nagai, K.]]
[[Category: Nagai K]]
[[Category: Oubridge, C.]]
[[Category: Oubridge C]]
[[Category: Teo, C H.]]
[[Category: Teo C-H]]
[[Category: hairpin loop]]
[[Category: overhanging base]]
[[Category: protein-rna complex]]
[[Category: single strand]]
 
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Retrieved from "https://proteopedia.org/1urn"