5npg: Difference between revisions
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==Solution structure of Drosophila melanogaster Loquacious dsRBD1== | |||
<StructureSection load='5npg' size='340' side='right'caption='[[5npg]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5npg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NPG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NPG FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5npg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5npg OCA], [https://pdbe.org/5npg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5npg RCSB], [https://www.ebi.ac.uk/pdbsum/5npg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5npg ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/X2J5X6_DROME X2J5X6_DROME] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
RNA interference defends against RNA viruses and retro-elements within an organism's genome. It is triggered by duplex siRNAs, of which one strand is selected to confer sequence-specificity to the RNA induced silencing complex (RISC). In Drosophila, Dicer-2 (Dcr-2) and the double-stranded RNA binding domain (dsRBD) protein R2D2 form the RISC loading complex (RLC) and select one strand of exogenous siRNAs according to the relative thermodynamic stability of base-pairing at either end. Through genome editing we demonstrate that Loqs-PD, the Drosophila homolog of human TAR RNA binding protein (TRBP) and a paralog of R2D2, forms an alternative RLC with Dcr-2 that is required for strand choice of endogenous siRNAs in S2 cells. Two canonical dsRBDs in Loqs-PD bind to siRNAs with enhanced affinity compared to miRNA/miRNA* duplexes. Structural analysis, NMR and biophysical experiments indicate that the Loqs-PD dsRBDs can slide along the RNA duplex to the ends of the siRNA. A moderate but notable binding preference for the thermodynamically more stable siRNA end by Loqs-PD alone is greatly amplified in complex with Dcr-2 to initiate strand discrimination by asymmetry sensing in the RLC. | |||
Molecular basis for asymmetry sensing of siRNAs by the Drosophila Loqs-PD/Dcr-2 complex in RNA interference.,Tants JN, Fesser S, Kern T, Stehle R, Geerlof A, Wunderlich C, Juen M, Hartlmuller C, Bottcher R, Kunzelmann S, Lange O, Kreutz C, Forstemann K, Sattler M Nucleic Acids Res. 2017 Oct 13. doi: 10.1093/nar/gkx886. PMID:29040648<ref>PMID:29040648</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5npg" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Drosophila melanogaster]] | |||
[[Category: Large Structures]] | |||
[[Category: Boettcher R]] | |||
[[Category: Fesser S]] | |||
[[Category: Foerstemann K]] | |||
[[Category: Geerlof A]] | |||
[[Category: Hartlmuller C]] | |||
[[Category: Kern T]] | |||
[[Category: Kreutz C]] | |||
[[Category: Kunzelmann S]] | |||
[[Category: Lange O]] | |||
[[Category: Sattler M]] | |||
[[Category: Stehle R]] | |||
[[Category: Tants J-N]] | |||
[[Category: Wunderlich C]] | |||
