5vik: Difference between revisions
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New page: '''Unreleased structure''' The entry 5vik is ON HOLD Authors: Pletnev Sergei Description: Crystal structure of monomeric near-infrared fluorescent protein miRFP703 [[Category: Unreleas... |
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==Crystal structure of monomeric near-infrared fluorescent protein miRFP703== | |||
<StructureSection load='5vik' size='340' side='right'caption='[[5vik]], [[Resolution|resolution]] 1.35Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5vik]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodopseudomonas_palustris Rhodopseudomonas palustris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VIK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VIK FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vik FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vik OCA], [https://pdbe.org/5vik PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vik RCSB], [https://www.ebi.ac.uk/pdbsum/5vik PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vik ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Brighter near-infrared (NIR) fluorescent proteins (FPs) are required for multicolor microscopy and deep-tissue imaging. Here, we present structural and biochemical analyses of three monomeric, spectrally distinct phytochrome-based NIR FPs, termed miRFPs. The miRFPs are closely related and differ by only a few amino acids, which define their molecular brightness, brightness in mammalian cells, and spectral properties. We have identified the residues responsible for the spectral red-shift, revealed a new chromophore bound simultaneously to two cysteine residues in the PAS and GAF domains in blue-shifted NIR FPs, and uncovered the importance of amino acid residues in the N-terminus of NIR FPs for their molecular and cellular brightness. The novel chromophore covalently links the N-terminus of NIR FPs with their C-terminal GAF domain, forming a topologically closed knot in the structure, and also contributes to the increased brightness. Based on our studies, we suggest a strategy to develop spectrally distinct NIR FPs with enhanced brightness. | |||
Designing brighter near-infrared fluorescent proteins: insights from structural and biochemical studies.,Baloban M, Shcherbakova DM, Pletnev S, Pletnev VZ, Lagarias JC, Verkhusha VV Chem Sci. 2017 Jun 1;8(6):4546-4557. doi: 10.1039/c7sc00855d. Epub 2017 May 4. PMID:28936332<ref>PMID:28936332</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Pletnev | <div class="pdbe-citations 5vik" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Rhodopseudomonas palustris]] | |||
[[Category: Pletnev S]] | |||
Latest revision as of 09:31, 23 October 2024
Crystal structure of monomeric near-infrared fluorescent protein miRFP703
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