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==Crystal structure of UDP-glucosyltransferase, UGT74F2, with UDP and salicylic acid==
==Crystal structure of UDP-glucosyltransferase, UGT74F2, with UDP and salicylic acid==
<StructureSection load='5u6m' size='340' side='right' caption='[[5u6m]], [[Resolution|resolution]] 2.57&Aring;' scene=''>
<StructureSection load='5u6m' size='340' side='right'caption='[[5u6m]], [[Resolution|resolution]] 2.57&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5u6m]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U6M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U6M FirstGlance]. <br>
<table><tr><td colspan='2'>[[5u6m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5U6M FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.568&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5u6s|5u6s]], [[5u6n|5u6n]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=SAL:2-HYDROXYBENZOIC+ACID'>SAL</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u6m OCA], [http://pdbe.org/5u6m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u6m RCSB], [http://www.ebi.ac.uk/pdbsum/5u6m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u6m ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5u6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u6m OCA], [https://pdbe.org/5u6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5u6m RCSB], [https://www.ebi.ac.uk/pdbsum/5u6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5u6m ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/U74F2_ARATH U74F2_ARATH]] Glycosyltransferase that glucosylates benzoic acid and derivatives. Substrate preference is benzoic acid > salicylic acid (SA) > 3-hydroxybenzoic acid > 4-hydroxybenzoic acid. Catalyzes the formation of both SA 2-O-beta-D-glucoside (SAG) and SA glucose ester (SGE). Has high affinity for the tryptophan precursor anthranilate. Catalyzes the formation of anthranilate glucose ester. Is the major source of this activity in the plant.<ref>PMID:11641410</ref> <ref>PMID:12475971</ref> <ref>PMID:17085977</ref> <ref>PMID:18226820</ref> <ref>PMID:18385129</ref>
[https://www.uniprot.org/uniprot/U74F2_ARATH U74F2_ARATH] Glycosyltransferase that glucosylates benzoic acid and derivatives. Substrate preference is benzoic acid > salicylic acid (SA) > 3-hydroxybenzoic acid > 4-hydroxybenzoic acid. Catalyzes the formation of both SA 2-O-beta-D-glucoside (SAG) and SA glucose ester (SGE). Has high affinity for the tryptophan precursor anthranilate. Catalyzes the formation of anthranilate glucose ester. Is the major source of this activity in the plant.<ref>PMID:11641410</ref> <ref>PMID:12475971</ref> <ref>PMID:17085977</ref> <ref>PMID:18226820</ref> <ref>PMID:18385129</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Salicylic acid (SA) is a signaling molecule utilized by plants in response to various stresses. Through conjugation with small organic molecules such as glucose, an inactive form of SA is generated which can be transported into and stored in plant vacuoles. In the model organism Arabidopsis thaliana, SA glucose conjugates are formed by two homologous enzymes (UGT74F1 and UGT74F2) that transfer glucose from UDP-glucose to SA. Despite being 77% identical and with conserved active site residues, these enzymes catalyze the formation of different products: UGT74F1 forms salicylic acid glucoside (SAG), while UGT74F2 forms primarily salicylic acid glucose ester (SGE). The position of the glucose on the aglycone determines how SA is stored, further metabolized, and contributes to a defense response. We determined the crystal structures of the UGT74F2 wild-type and T15S mutant enzymes, in different substrate/product complexes. On the basis of the crystal structures and the effect on enzyme activity of mutations in the SA binding site, we propose the catalytic mechanism of SGE and SAG formation and that SA binds to the active site in two conformations, with each enzyme selecting a certain binding mode of SA. Additionally, we show that two threonines are key determinants of product specificity.


Differences in salicylic acid glucose conjugations by UGT74F1 and UGT74F2 from Arabidopsis thaliana.,George Thompson AM, Iancu CV, Neet KE, Dean JV, Choe JY Sci Rep. 2017 Apr 20;7:46629. doi: 10.1038/srep46629. PMID:28425481<ref>PMID:28425481</ref>
==See Also==
 
*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5u6m" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Choe, J]]
[[Category: Arabidopsis thaliana]]
[[Category: Dean, J V]]
[[Category: Large Structures]]
[[Category: Iancu, C V]]
[[Category: Choe J]]
[[Category: Thompson, A M.George]]
[[Category: Dean JV]]
[[Category: Salicylic acid]]
[[Category: George Thompson AM]]
[[Category: Salicylic acid ester]]
[[Category: Iancu CV]]
[[Category: Salicylic acid glucoside]]
[[Category: Transferase]]
[[Category: Udp-glucosyltransferase]]

Latest revision as of 15:45, 6 March 2024

Crystal structure of UDP-glucosyltransferase, UGT74F2, with UDP and salicylic acid

5u6m, resolution 2.57Å

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