5nxt: Difference between revisions
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New page: '''Unreleased structure''' The entry 5nxt is ON HOLD Authors: Description: Category: Unreleased Structures |
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==Wobble base paired RNA double helix== | |||
<StructureSection load='5nxt' size='340' side='right'caption='[[5nxt]], [[Resolution|resolution]] 1.38Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5nxt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NXT FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.379Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nxt OCA], [https://pdbe.org/5nxt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nxt RCSB], [https://www.ebi.ac.uk/pdbsum/5nxt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nxt ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Wobble base pairs are critical in various physiological functions and have been linked to local structural perturbations in double helical structures of nucleic acids. We report a 1.38-A resolution crystal structure of an antiparallel octadecamer RNA double helix in overall A conformation, which includes a unique, central stretch of six consecutive wobble base pairs (W helix) with two G.U and four rare C.A(+) wobble pairs. Four adenines within the W helix are N1-protonated and wobble-base-paired with the opposing cytosine through two regular hydrogen bonds. Combined with the two G.U pairs, the C.A(+) base pairs facilitate formation of a half turn of W-helical RNA flanked by six regular Watson-Crick base pairs in standard A conformation on either side. RNA melting experiments monitored by differential scanning calorimetry, UV and circular dichroism spectroscopy demonstrate that the RNA octadecamer undergoes a pH-induced structural transition which is consistent with the presence of a duplex with C.A(+) base pairs at acidic pH. Our crystal structure provides a first glimpse of an RNA double helix based entirely on wobble base pairs with possible applications in RNA or DNA nanotechnology and pH biosensors. | |||
A Novel Form of RNA Double Helix Based on G.U and C.A(+) Wobble Base Pairing.,Garg A, Heinemann U RNA. 2017 Nov 9. pii: rna.064048.117. doi: 10.1261/rna.064048.117. PMID:29122970<ref>PMID:29122970</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5nxt" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Garg A]] | |||
[[Category: Heinemann U]] | |||
Latest revision as of 12:15, 22 November 2023
Wobble base paired RNA double helix
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