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==Cryo-EM structure of the ATP-bound Vps4 mutant-E233Q complex with Vta1 (masked)==
==Cryo-EM structure of the ATP-bound Vps4 mutant-E233Q complex with Vta1 (masked)==
<StructureSection load='5xmk' size='340' side='right' caption='[[5xmk]], [[Resolution|resolution]] 4.18&Aring;' scene=''>
<SX load='5xmk' size='340' side='right' viewer='molstar' caption='[[5xmk]], [[Resolution|resolution]] 4.18&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5xmk]] is a 14 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XMK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XMK FirstGlance]. <br>
<table><tr><td colspan='2'>[[5xmk]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XMK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XMK FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.18&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xmk OCA], [http://pdbe.org/5xmk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xmk RCSB], [http://www.ebi.ac.uk/pdbsum/5xmk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xmk ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xmk OCA], [https://pdbe.org/5xmk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xmk RCSB], [https://www.ebi.ac.uk/pdbsum/5xmk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xmk ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/VPS4_YEAST VPS4_YEAST]] Involved in the transport of biosynthetic membrane proteins from the prevacuolar/endosomal compartment to the vacuole. Required for multivesicular body (MVB) protein sorting. Catalyzes the ATP-dependent dissociation of class E VPS proteins from endosomal membranes, such as the disassembly of the ESCRT-III complex.<ref>PMID:11329380</ref> <ref>PMID:9155008</ref> <ref>PMID:9606181</ref> [[http://www.uniprot.org/uniprot/VTA1_YEAST VTA1_YEAST]] Has a role in the formation of the multivesicular body (MVB). Required for the sorting of lipids to form intralumenal vesicles and for fluid-phase transport to the vacuole. Required for sorting the plasma membrane proteins STE2 and STE3 into the MVB. Acts a cofactor of VSP4, promotes the oligomerization of VPS4 and stimulates its ATPase activity by 6- to 8-fold.<ref>PMID:12953057</ref> <ref>PMID:14701806</ref> <ref>PMID:16505166</ref> <ref>PMID:16601096</ref> 
[https://www.uniprot.org/uniprot/VPS4_YEAST VPS4_YEAST] Involved in the transport of biosynthetic membrane proteins from the prevacuolar/endosomal compartment to the vacuole. Required for multivesicular body (MVB) protein sorting. Catalyzes the ATP-dependent dissociation of class E VPS proteins from endosomal membranes, such as the disassembly of the ESCRT-III complex.<ref>PMID:11329380</ref> <ref>PMID:9155008</ref> <ref>PMID:9606181</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The cellular ESCRT-III (endosomal sorting complex required for transport-III) and Vps4 (vacuolar protein sorting 4) comprise a common machinery that mediates a variety of membrane remodelling events. Vps4 is essential for the machinery function by using the energy from ATP hydrolysis to disassemble the ESCRT-III polymer into individual proteins. Here, we report the structures of the ATP-bound Vps4E233Q hexamer and its complex with the cofactor Vta1 (vps twenty associated 1) at resolutions of 3.9 and 4.2 A, respectively, determined by electron cryo-microscopy. Six Vps4E233Q subunits in both assemblies exhibit a spiral-shaped ring-like arrangement. Locating at the periphery of the hexameric ring, Vta1 dimer bridges two adjacent Vps4 subunits by two different interaction modes to promote the formation of the active Vps4 hexamer during ESCRT-III filament disassembly. The structural findings, together with the structure-guided biochemical and single-molecule analyses, provide important insights into the process of the ESCRT-III polymer disassembly by Vps4.


Cryo-EM structures of the ATP-bound Vps4E233Q hexamer and its complex with Vta1 at near-atomic resolution.,Sun S, Li L, Yang F, Wang X, Fan F, Yang M, Chen C, Li X, Wang HW, Sui SF Nat Commun. 2017 Jul 17;8:16064. doi: 10.1038/ncomms16064. PMID:28714467<ref>PMID:28714467</ref>
==See Also==
 
*[[Vacuolar protein sorting-associated protein 3D structures|Vacuolar protein sorting-associated protein 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5xmk" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Fan, F]]
[[Category: Large Structures]]
[[Category: Li, L]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Li, X]]
[[Category: Fan F]]
[[Category: Sui, S]]
[[Category: Li L]]
[[Category: Sun, S]]
[[Category: Li X]]
[[Category: Wang, H]]
[[Category: Sui S]]
[[Category: Wang, X]]
[[Category: Sun S]]
[[Category: Yang, F]]
[[Category: Wang H]]
[[Category: Atpase]]
[[Category: Wang X]]
[[Category: Escrtiii]]
[[Category: Yang F]]
[[Category: Protein transport]]
[[Category: Vps4]]
[[Category: Vta1]]

Latest revision as of 10:19, 27 March 2024

Cryo-EM structure of the ATP-bound Vps4 mutant-E233Q complex with Vta1 (masked)

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