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==Crystal structure of the restriction endonuclease BglI (e.c.3.1.21.4) bound to its dna recognition sequence==
==Crystal structure of the restriction endonuclease BglI (e.c.3.1.21.4) bound to its dna recognition sequence==
<StructureSection load='1dmu' size='340' side='right' caption='[[1dmu]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='1dmu' size='340' side='right'caption='[[1dmu]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1dmu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DMU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1DMU FirstGlance]. <br>
<table><tr><td colspan='2'>[[1dmu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DMU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DMU FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1dmu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dmu OCA], [http://pdbe.org/1dmu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1dmu RCSB], [http://www.ebi.ac.uk/pdbsum/1dmu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1dmu ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dmu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dmu OCA], [https://pdbe.org/1dmu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dmu RCSB], [https://www.ebi.ac.uk/pdbsum/1dmu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dmu ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/T2B1_BACIU T2B1_BACIU] A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-GCCNNNNNGGC-3' and cleaves before N-8.<ref>PMID:9736624</ref> <ref>PMID:12654995</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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==See Also==
==See Also==
*[[Endonuclease|Endonuclease]]
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Bacillus subtilis]]
[[Category: Greci, J]]
[[Category: Large Structures]]
[[Category: Lunnen, K]]
[[Category: Greci J]]
[[Category: Newman, M]]
[[Category: Lunnen K]]
[[Category: Phillips, S E.V]]
[[Category: Newman M]]
[[Category: Schildkraut, I]]
[[Category: Phillips SEV]]
[[Category: Wilson, G]]
[[Category: Schildkraut I]]
[[Category: A:a mismatch]]
[[Category: Wilson G]]
[[Category: Active site calcium ion]]
[[Category: Alpha/beta structure]]
[[Category: Hydrolase-dna complex]]
[[Category: Protein-dna complex]]
Retrieved from "https://proteopedia.org/1dmu"