Resolvase: Difference between revisions

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<StructureSection load='' size='340' side='right' caption='Yeast Holliday junction resolvase dimer complex with sulfate, [[1kcf]]' scene='' >
<StructureSection load='1kcf' size='340' side='right' caption='Yeast Holliday junction resolvase dimer complex with sulfate, [[1kcf]]' scene='' >
== Function ==
== Function ==
[[Resolvase]] or '''recombinase''' (Rec) is a nuclease which is involved in DNA recombination. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase.  
[[Resolvase]] or '''recombinase''' (Rec) is a nuclease which is involved in DNA recombination. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase.  


*'''Holliday junction resolvase''' (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands<ref>PMID:19442245</ref>. <br />  
*'''Holliday junction resolvase''' (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands<ref>PMID:19442245</ref>. <br />  
*'''Tyr-recombinases''' include Cre which recombines loxP sites, FLP and lambda integrase (LamInt). <br />
*'''Tyr-recombinases''' include '''Cre''' which recombines loxP sites, FLP and lambda integrase (LamInt). <br />
*'''Ser-recombinases''' (S-rec) include gamma-delta resolvase  (GDR), Tn3 resolvase and phiC31 integrase.<br />
*'''Ser-recombinases''' (S-rec) include gamma-delta resolvase  (GDR), Tn3 resolvase and phiC31 integrase.<br />
*'''RadA recombinase''' promotes DNA recombination.  Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID:19143611</ref>.  <br />
*'''RadA recombinase''' promotes DNA recombination.  Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID:19143611</ref>.  <br />
*'''RadB recombinase''' is a paralogue of RadA assisting in RadA-mediated strand exchange <ref>PMID:28501701</ref>.  <br />
*'''Hin-recombinase''' (HRec) is a protein of ''Salmonella''.  HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes.  The inversion changes the these genes' expressions<ref>PMID:15454079</ref>.
*'''Hin-recombinase''' (HRec) is a protein of ''Salmonella''.  HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes.  The inversion changes the these genes' expressions<ref>PMID:15454079</ref>.
</StructureSection>
*'''Tre recombinase''' recognizes a target sequence of the HIV virus resulting in excision and eradication of the provirus from infected human cells <ref>PMID:19066582</ref>.  <br />
 
==3D structures of resolvase==
==3D structures of resolvase==
[[Resolvase 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
</StructureSection>
{{#tree:id=OrganizedByTopic|openlevels=0|
 
* Holliday junction resolvase
 
**[[2wiw]], [[2wiz]], [[2wj0]] – AfHJR+DNA – ''Archaeoglobus fulgidus''<br />
**[[2wcw]], [[2wcz]] – AfHJR (mutant)<br />
**[[2h8c]] – EcHJR RusA (mutant) + DNA – ''Escherichia coli''<br />
**[[1gdt]] - EcHJR +DNA<br />
**[[2h8e]] - EcHJR RusA (mutant)<br />
**[[1q8r]] – EcHJR RusA<br />
**[[1hjr]] – EcHJR RuvC<br />
**[[4ep4]] – TtHJR RuvC – ''Thermus thermophilus''<br />
**[[4ep5]] – TtHJR RuvC (mutant)<br />
**[[1zp7]] – BsHJR RecU – ''Bacillus subtilis''<br />
**[[5fdk]] - BsHJR RecU (mutant) + DNA<br />
**[[1ob8]], [[1ob9]], [[1hh1]], [[2eo0]] – SsHJR – ''Sulfolobus solfataricus''<br />
**[[4tkd]], [[4tkk]] - SsHJR (mutant) - <br />
**[[2fco]] – HJR – ''Geobacillus kaustophilus''<br />
**[[1ipi]], [[1gef]] – PfHJR – ''Pyrococcus furiosus''<br />
**[[1kcf]] – HJR – fission yeast<br />
**[[1bvs]] - RuvA - ''Mycobacterium leprae''<br />
**[[5e6f]] - HJR - canarypox virus<br />
 
* Ser-recombinase
 
**[[2rsl]] - EcGDR<br />
**[[1hx7]] – EcGDR N-terminal – NMR<br />
**[[1ght]], [[1res]], [[1ret]], [[1ght]] – EcGDR catalytic domain – NMR<br />
**[[1gdr]], [[1hx7]] - EcGDR catalytic domain<br />
**[[2gm4]], [[1zr2]], [[1zr4]], [[1gdt]] – EcGDR+DNA<br />
**[[2gm5]] – EcGDR (mutant)<br />
**[[3guv]] – Sp-site-specific recombinase – ''Streptococcus pneumoniae''<br />
**[[2r0q]] – SaS-rec+DNA – ''Staphylococcus aureus''<br />
**[[3pkz]] - SaS-sin (mutant)<br />
**[[3lhf]] – SsS-rec<br />
 
* Tyr-recombinase
 
**[[3etl]], [[3ew9]], [[3ewa]], [[2i1q]] – MmRadA Rec+AMPPNP+ion – ''Methanococcus maripaludis''<br />
**[[2b21]] - MmRadA Rec+AMPPNP<br />
**[[2zub]], [[2zuc]], [[2zud]], [[2dfl]] – SsRadA<br />
**[[2cvf]], [[2cvh]] – RadB Rec – ''Thermococcus kodakarensis''<br />
**[[2f1h]], [[2fpl]], [[2fpm]] - MvRec+AMPPNP+K – ''Methanococcus voltae''<br />
**[[2f1i]] – MvRec+AMPPNP<br />
**[[2f1j]], [[2fpk]] - MvRec+ADP<br />
**[[1a0p]] – EcXerD Rec<br />
**[[5dcf]] - EcXerD C-terminal/FTSK<br />
**[[5hxy]] - XerD - ''Thermoplasma acidophilum''<br />
**[[5jjv]], [[5jk0]] - XerH - ''Helicobacter pylori''<br />
 
* Cre recombinase
 
**[[3mgv]], [[3c28]], [[3c29]], [[2hof]], [[2hoi]], [[1xns]], [[1xo0]], [[1pvp]], [[1pvq]], [[1pvr]], [[1nzb]], [[1ouq]], [[1q3u]], [[1q3v]], [[1ma7]], [[1kbu]], [[1drg]], [[1f44]], [[2crx]], [[3crx]], [[4crx]], [[5crx]], [[1crx]] – EpCre+DNA – Enterobacteria phage PI<br />
 
*Hin recombinase
 
**[[1hcr]], [[1ijw]], [[1ijj6]], [[1jj8]], [[1jko]], [[1jkp]], [[1jkq]], [[1jkr]] – HRec + DNA – synthetic <br />
 
*RadA recombinase
 
**[[2zub]], [[2zuc]], [[2zud]], [[2dfl]] – SsRadA  <br />
**[[4dc9]] – MvRadA C-terminal domain  <br />
**[[2i1q]] – MvRadA (mutant)  <br />
**[[1pzn]], [[5fos]], [[5fot]], [[5fou]], [[5fox]], [[5fpk]], [[5fov]], [[5fow]] – PfRadA  <br />
**[[4a6p]] – PfRadA ATPase domain (mutant)  <br />
**[[5lkq]] - SpRadA protease domain<br />
**[[5j4l]], [[5jec]], [[5jed]], [[5jee]], [[5kdd]], [[5l8v]], [[5lb2]], [[5lb4]], [[5lbi]], [[5jfg]] - PfRadA (mutant)<br />
**[[5h45]] - TtRadA C-terminal<br />
 
*RadA recombinase complex
 
**[[5fot]], [[5fou]], [[5fov]], [[5fow]], [[5fpk]], [[4b3b]] – PfRadA ATPase domain + tetrapeptide <br />
**[[4d6p]] – PfRadA ATPase domain (mutant) + AMPPNP <br />
**[[4uqo]] – PfRadA ATPase domain (mutant) + ADP <br />
**[[4b2i]] – PfRadA ATPase domain (mutant) + indazole <br />
**[[4b2l]], [[4b35]] – PfRadA ATPase domain (mutant) + methylester tryplophan <br />
**[[4b32]], [[4b33]] – PfRadA ATPase domain (mutant) + naphthol <br />
**[[4b34]] – PfRadA ATPase domain (mutant) + aminobenzothiazole <br />
**[[4b3c]], [[4b3d]], [[5j4h]], [[5j4k]] – PfRadA ATPase domain (mutant) + indole derivative <br />
**[[4a6x]] – PfRadA ATPase domain (mutant) + ATP <br />
**[[3ntu]], [[2gdj]] – MvRadA  + AMPPNP <br />
**[[2b21]], [[2f1h]], [[2f1i]], [[2fpl]], [[2fpm]] – MvRadA  (mutant) + AMPPNP <br />
**[[3fyh]], [[2f1j]], [[2fpk]] – MvRadA  (mutant) + ADP <br />
**[[3etl]], [[3ew9]], [[3ewa]] – MmRadA  (mutant) + AMPPNP <br />
**[[5lkm]] - SpRadA + TDP<br />
 
*RadB recombinase
 
**[[2cvf]], [[2cvh]] – TkRadB  <br />
 
* Integrase
 
**[[2a3v]] – Rec INTI4+DNA – ''Vibrio cholerae''<br />
**[[3bvp]] – Integrase N-terminal – Lactococcus phage<br />
**[[2oxo]] – LamInt binding domain – phage<br />
**[[1z19]] – EpLamInt core binding +catalytic domain+DNA<br />
**[[1z1b]], [[1z1g]], [[1p7d]] – EpLamInt+DNA<br />
**[[1kjk]] – EpLamInt N-terminal – NMR<br />
**[[1ae9]] – EpLamInt catalytic core<br />
**[[1p4e]], [[1m6x]] – yFLP (mutant)+DNA – yeast<br />
**[[1flo]] - yFLP +DNA<br />
**[[1aih]] – HP1 integrase catalytic domain - bacteriophage<br />
**[[3nrw]] – INT/site-specific recombinase N-terminal – ''Haloarcula marismortui''<br />
**[[3jtz]] – INT arm-type binding domain – ''Yersinia pestis''<br />
**[[3ju0]] – HPI INT arm-type binding domain – ''Pectobacterium atrosepticum''<br />
**[[2kkp]] – INT SAM-like domain – ''Moorella thermoacetica'' – NMR<br />
**[[2kkv]] – INT fragment – ''Salmonella enterica'' – NMR<br />
**[[2khq]] - INT fragment – ''Staphylococcus saprophyticus'' – NMR<br />
**[[2wcc]] – λINT DBD+ DNA – Enterobacteria phage λ - NMR<br />
**[[2khv]], [[2kj5]] – INT residues 102-199 – ''Nitrosospira multiformis'' – NMR<br />
**[[5udo]] - INT coiled-coil domain - ''Listeria innocua''<br />
**[[5frn]] - INT + DNA - human spumaretrovirus<br />


*Recombinase A (RecA) see [[Recombinase A]]
}}
==References==
==References==
<references />
<references />


[[Category:Topic Page]]
[[Category:Topic Page]]
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