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| [[Image:1x22.gif|left|200px]]
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| {{Structure
| | ==Solution structure of a novel moricin analogue, an antibacterial peptide from a lepidopteran insect, Spodoptera litura== |
| |PDB= 1x22 |SIZE=350|CAPTION= <scene name='initialview01'>1x22</scene>
| | <StructureSection load='1x22' size='340' side='right'caption='[[1x22]]' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND=
| | <table><tr><td colspan='2'>[[1x22]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Spodoptera_litura Spodoptera litura]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X22 FirstGlance]. <br> |
| |ACTIVITY= | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| |GENE=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x22 OCA], [https://pdbe.org/1x22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x22 RCSB], [https://www.ebi.ac.uk/pdbsum/1x22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x22 ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=
| | == Function == |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x22 OCA], [http://www.ebi.ac.uk/pdbsum/1x22 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1x22 RCSB]</span>
| | [https://www.uniprot.org/uniprot/Q7YZB4_SPOLT Q7YZB4_SPOLT] |
| }}
| | __TOC__ |
| | | </StructureSection> |
| '''Solution structure of a novel moricin analogue, an antibacterial peptide from a lepidopteran insect, Spodoptera litura'''
| | [[Category: Large Structures]] |
| | | [[Category: Spodoptera litura]] |
| | | [[Category: Asaoka A]] |
| ==Overview==
| | [[Category: Hemmi H]] |
| An antibacterial peptide was isolated from a lepidopteran insect, Spodoptera litura. The molecular mass of this peptide was determined to be 4489.55 by matrix assisted laser desorption/ionization-time of flight mass (MALDI-TOF MS) spectrometry. The peptide consists of 42 amino acids and the sequence has 69-98% identity to those of moricin-related peptides, antibacterial peptides from lepidopetran insects. Thus, the peptide was designated S. litura (Sl) moricin. Sl moricin showed a broad antibacterial spectrum against Gram-positive and negative bacteria. Sl moricin gene was inducible by bacterial injection and expressed tissue-specifically in the fat body and hemocytes. Furthermore, the solution structure of Sl moricin was determined by two-dimensional (2D) 1H-nuclear magnetic resonance (NMR) spectroscopy and hybrid distance geometry-simulated annealing calculation. The tertiary structure revealed a long alpha-helix containing eight turns along nearly the full length of the peptide like that of moricin, confirming that Sl moricin is a new moricin-like antibacterial peptide. These results suggest that moricin is present not only in B. mori but also in other lepidopteran insects forming a gene family.
| | [[Category: Minami M]] |
| | | [[Category: Oizumi Y]] |
| ==About this Structure==
| | [[Category: Yamakawa M]] |
| 1X22 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X22 OCA].
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| ==Reference== | |
| Isolation, gene expression and solution structure of a novel moricin analogue, antibacterial peptide from a lepidopteran insect, Spodoptera litura., Oizumi Y, Hemmi H, Minami M, Asaoka A, Yamakawa M, Biochim Biophys Acta. 2005 Aug 31;1752(1):83-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16115804 16115804]
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| [[Category: Single protein]] | |
| [[Category: Asaoka, A.]] | |
| [[Category: Hemmi, H.]] | |
| [[Category: Minami, M.]] | |
| [[Category: Oizumi, Y.]] | |
| [[Category: Yamakawa, M.]] | |
| [[Category: antibacterial peptide]]
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| [[Category: gene expression]]
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| [[Category: insect immunity]]
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| [[Category: moricin]]
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| [[Category: nmr]]
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| [[Category: purification]]
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| [[Category: solution structure]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:43:54 2008''
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