5omt: Difference between revisions
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New page: '''Unreleased structure''' The entry 5omt is ON HOLD until Paper Publication Authors: Basle, A., Lewis, R.J. Description: Endonuclease NucB Category: Unreleased Structures [[Catego... |
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==Endonuclease NucB== | |||
<StructureSection load='5omt' size='340' side='right'caption='[[5omt]], [[Resolution|resolution]] 1.35Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5omt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OMT FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5omt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5omt OCA], [https://pdbe.org/5omt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5omt RCSB], [https://www.ebi.ac.uk/pdbsum/5omt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5omt ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/F1BV52_BACLI F1BV52_BACLI] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacterial biofilms are a complex architecture of cells that grow on moist interfaces, and are held together by a molecular glue of extracellular proteins, sugars and nucleic acids. Biofilms are particularly problematic in human healthcare as they can coat medical implants and are thus a potential source of disease. The enzymatic dispersal of biofilms is increasingly being developed as a new strategy to treat this problem. Here, we have characterized NucB, a biofilm-dispersing nuclease from a marine strain of Bacillus licheniformis, and present its crystal structure together with the biochemistry and a mutational analysis required to confirm its active site. Taken together, these data support the categorization of NucB into a unique subfamily of the betabetaalpha metal-dependent non-specific endonucleases. Understanding the structure and function of NucB will facilitate its future development into an anti-biofilm therapeutic agent. | |||
Crystal structure of NucB, a biofilm-degrading endonuclease.,Basle A, Hewitt L, Koh A, Lamb HK, Thompson P, Burgess JG, Hall MJ, Hawkins AR, Murray H, Lewis RJ Nucleic Acids Res. 2017 Nov 20. pii: 4641913. doi: 10.1093/nar/gkx1170. PMID:29165717<ref>PMID:29165717</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5omt" style="background-color:#fffaf0;"></div> | ||
[[Category: Basle | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Bacillus licheniformis]] | |||
[[Category: Large Structures]] | |||
[[Category: Basle A]] | |||
[[Category: Lewis RJ]] | |||
