5oph: Difference between revisions
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The | ==G-quadruplex structure of DNA oligonucleotide containing GGGGCC repeats linked to ALS and FTD== | ||
<StructureSection load='5oph' size='340' side='right'caption='[[5oph]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5oph]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OPH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OPH FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGM:8-BROMO-2-DEOXYGUANOSINE-5-MONOPHOSPHATE'>BGM</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5oph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oph OCA], [https://pdbe.org/5oph PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5oph RCSB], [https://www.ebi.ac.uk/pdbsum/5oph PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5oph ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Most frequent genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), is a largely increased number of d(G4C2)n*(G2C4)n repeats located in the non-coding region of C9orf72 gene. Non-canonical structures, including G-quadruplexes, formed within expanded repeats have been proposed to drive repeat expansion and pathogenesis of ALS and FTD. Oligonucleotide d[(G4C2)3G4], which represents the shortest oligonucleotide model of d(G4C2) repeats with the ability to form a unimolecular G-quadruplex, forms two major G-quadruplex structures in addition to several minor species which coexist in solution with K+ ions. Herein, we used solution-state NMR to determine the high-resolution structure of one of the major G-quadruplex species adopted by d[(G4C2)3G4]. Structural characterization of the G-quadruplex named AQU was facilitated by a single substitution of dG with 8Br-dG at position 21 and revealed an antiparallel fold composed of four G-quartets and three lateral C-C loops. The G-quadruplex exhibits high thermal stability and is favored kinetically and under slightly acidic conditions. An unusual structural element distinct from a C-quartet is observed in the structure. Two C*C base pairs are stacked on the nearby G-quartet and are involved in a dynamic equilibrium between symmetric N3-amino and carbonyl-amino geometries and protonated C+*C state. | |||
NMR structure of a G-quadruplex formed by four d(G4C2) repeats: insights into structural polymorphism.,Brcic J, Plavec J Nucleic Acids Res. 2018 Oct 2. pii: 5114260. doi: 10.1093/nar/gky886. PMID:30277522<ref>PMID:30277522</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Brcic | <div class="pdbe-citations 5oph" style="background-color:#fffaf0;"></div> | ||
[[Category: Plavec | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Brcic J]] | |||
[[Category: Plavec J]] | |||
Latest revision as of 16:57, 13 December 2023
G-quadruplex structure of DNA oligonucleotide containing GGGGCC repeats linked to ALS and FTD
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