5owp: Difference between revisions
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==Crystal structure of glycopeptide "GVTSAfPDT*RPAP" in complex with scFv-SM3== | |||
<StructureSection load='5owp' size='340' side='right'caption='[[5owp]], [[Resolution|resolution]] 1.85Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5owp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OWP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OWP FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4FB:(4S)-4-FLUORO-L-PROLINE'>4FB</scene>, <scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5owp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5owp OCA], [https://pdbe.org/5owp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5owp RCSB], [https://www.ebi.ac.uk/pdbsum/5owp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5owp ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/LV1D_MOUSE LV1D_MOUSE] [https://www.uniprot.org/uniprot/HVM32_MOUSE HVM32_MOUSE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A structure-based design of a new generation of tumor-associated glycopeptides with improved affinity against two anti-MUC1 antibodies is described. These unique antigens feature a fluorinated proline residue, such as a (4S)-4-fluoro-l-proline or 4,4-difluoro-l-proline, at the most immunogenic domain. Binding assays using biolayer interferometry reveal 3-fold to 10-fold affinity improvement with respect to the natural (glyco)peptides. According to X-ray crystallography and MD simulations, the fluorinated residues stabilize the antigen-antibody complex by enhancing key CH/pi interactions. Interestingly, a notable improvement in detection of cancer-associated anti-MUC1 antibodies from serum of patients with prostate cancer is achieved with the non-natural antigens, which proves that these derivatives can be considered better diagnostic tools than the natural antigen for prostate cancer. | |||
The Use of Fluoroproline in MUC1 Antigen Enables Efficient Detection of Antibodies in Patients with Prostate Cancer.,Somovilla VJ, Bermejo IA, Albuquerque IS, Martinez-Saez N, Castro-Lopez J, Garcia-Martin F, Companon I, Hinou H, Nishimura SI, Jimenez-Barbero J, Asensio JL, Avenoza A, Busto JH, Hurtado-Guerrero R, Peregrina JM, Bernardes GJL, Corzana F J Am Chem Soc. 2017 Dec 12. doi: 10.1021/jacs.7b09447. PMID:29166012<ref>PMID:29166012</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5owp" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Antibody 3D structures|Antibody 3D structures]] | ||
[[Category: | *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] | ||
[[Category: | *[[3D structures of non-human antibody|3D structures of non-human antibody]] | ||
[[Category: | == References == | ||
[[Category: Castro-Lopez | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Hurtado-Guerrero | [[Category: Mus musculus]] | ||
[[Category: | [[Category: Synthetic construct]] | ||
[[Category: | [[Category: Albuquerque IS]] | ||
[[Category: | [[Category: Asensio JL]] | ||
[[Category: | [[Category: Avenoza A]] | ||
[[Category: Bermejo IA]] | |||
[[Category: Bernardes GJ]] | |||
[[Category: Busto JH]] | |||
[[Category: Castro-Lopez J]] | |||
[[Category: Corzana F]] | |||
[[Category: Garcia-Martin F]] | |||
[[Category: Hinou H]] | |||
[[Category: Hurtado-Guerrero R]] | |||
[[Category: Jimenez-Barbero J]] | |||
[[Category: Martinez-Saez N]] | |||
[[Category: Nishimura S]] | |||
[[Category: Peregrina JM]] | |||
[[Category: Somovilla VJ]] | |||
Latest revision as of 01:26, 28 December 2023
Crystal structure of glycopeptide "GVTSAfPDT*RPAP" in complex with scFv-SM3
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