5oxw: Difference between revisions
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New page: '''Unreleased structure''' The entry 5oxw is ON HOLD Authors: Aparicio, D., Perez-Luque, R., Ribo, M., Fita, I. Description: Intein Category: Unreleased Structures [[Category: Pere... |
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The | ==Structure of NeqN from Nanoarchaeum equitans== | ||
<StructureSection load='5oxw' size='340' side='right'caption='[[5oxw]], [[Resolution|resolution]] 2.61Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5oxw]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Nanoarchaeum_equitans Nanoarchaeum equitans] and [https://en.wikipedia.org/wiki/Nanoarchaeum_equitans_Kin4-M Nanoarchaeum equitans Kin4-M]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OXW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OXW FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.61Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5oxw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oxw OCA], [https://pdbe.org/5oxw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5oxw RCSB], [https://www.ebi.ac.uk/pdbsum/5oxw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5oxw ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q74N74_NANEQ Q74N74_NANEQ] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Split inteins are expressed as two separated subunits (N-intein and C-intein) fused to the corresponding exteins. The specific association of both intein subunits precedes protein splicing, which results in excision of the intein subunits and in ligation, by a peptide bond, of the concomitant exteins. Catalytically active intein precursors are typically too reactive for crystallization or even isolation. Neq pol is the trans-intein of the B-type DNA polymerase I split gene from hyperthermophile Nanoarchaeum equitans. We have determined the crystal structures of both the isolated NeqN and the complex of NeqN and NeqC subunits carrying the wild-type sequences, including the essential catalytic residues Ser1 and Thr+1, in addition to seven and three residues of the N- and C-exteins, respectively. These structures provide detailed information on the unique oxyester chemistry of the splicing mechanism of Neq pol and of the extensive rearrangements that occur in NeqN during the association step. | |||
Structural Insights into Subunits Assembly and the Oxyester Splicing Mechanism of Neq pol Split Intein.,Gordo V, Aparicio D, Perez-Luque R, Benito A, Vilanova M, Uson I, Fita I, Ribo M Cell Chem Biol. 2018 Jul 19;25(7):871-879.e2. doi:, 10.1016/j.chembiol.2018.04.008. Epub 2018 May 10. PMID:29754955<ref>PMID:29754955</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5oxw" style="background-color:#fffaf0;"></div> | ||
[[Category: Aparicio | == References == | ||
[[Category: Fita | <references/> | ||
[[Category: Ribo | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Nanoarchaeum equitans]] | |||
[[Category: Nanoarchaeum equitans Kin4-M]] | |||
[[Category: Aparicio D]] | |||
[[Category: Fita I]] | |||
[[Category: Perez-Luque R]] | |||
[[Category: Ribo M]] | |||
Latest revision as of 01:28, 28 December 2023
Structure of NeqN from Nanoarchaeum equitans
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