5y31: Difference between revisions

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~~'''Unreleased structure'''~~

~~The entry~~ 5y31 is ~~ON HOLD until Paper~~ Publication

==Crystal structure of human LGI1-ADAM22 complex==

== Structural highlights ==

<table><tr><td colspan='2'>[[5y31]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Y31 FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 7.125Å</td></tr>

<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5y31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y31 OCA], [https://pdbe.org/5y31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5y31 RCSB], [https://www.ebi.ac.uk/pdbsum/5y31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5y31 ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/ADA22_HUMAN ADA22_HUMAN] Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1.<ref>PMID:12589811</ref> <ref>PMID:15882968</ref> <ref>PMID:16385342</ref>

== Publication Abstract from PubMed ==

Epilepsy is a common brain disorder throughout history. Epilepsy-related ligand-receptor complex, LGI1-ADAM22, regulates synaptic transmission and has emerged as a determinant of brain excitability, as their mutations and acquired LGI1 autoantibodies cause epileptic disorders in human. Here, we report the crystal structure of human LGI1-ADAM22 complex, revealing a 2:2 heterotetrameric assembly. The hydrophobic pocket of the C-terminal epitempin-repeat (EPTP) domain of LGI1 binds to the metalloprotease-like domain of ADAM22. The N-terminal leucine-rich repeat and EPTP domains of LGI1 mediate the intermolecular LGI1-LGI1 interaction. A pathogenic R474Q mutation of LGI1, which does not exceptionally affect either the secretion or the ADAM22 binding, is located in the LGI1-LGI1 interface and disrupts the higher-order assembly of the LGI1-ADAM22 complex in vitro and in a mouse model for familial epilepsy. These studies support the notion that the LGI1-ADAM22 complex functions as the trans-synaptic machinery for precise synaptic transmission.

~~Authors~~:

Structural basis of epilepsy-related ligand-receptor complex LGI1-ADAM22.,Yamagata A, Miyazaki Y, Yokoi N, Shigematsu H, Sato Y, Goto-Ito S, Maeda A, Goto T, Sanbo M, Hirabayashi M, Shirouzu M, Fukata Y, Fukata M, Fukai S Nat Commun. 2018 Apr 18;9(1):1546. doi: 10.1038/s41467-018-03947-w. PMID:29670100<ref>PMID:29670100</ref>

~~Description~~:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased~~ Structures]]

</div>

==See Also==

*[[A Disintegrin And Metalloproteinase 3D structures|A Disintegrin And Metalloproteinase 3D structures]]

== References ==

__TOC__

</StructureSection>

[[Category: Homo sapiens]]

[[Category: Large Structures]]

[[Category: Fukai S]]

[[Category: Yamagata A]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 5y31 is ON HOLD  until Paper Publication
+==Crystal structure of human LGI1-ADAM22 complex==
+<StructureSection load='5y31' size='340' side='right'caption='[[5y31]], [[Resolution|resolution]] 7.12&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5y31]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Y31 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 7.125&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5y31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y31 OCA], [https://pdbe.org/5y31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5y31 RCSB], [https://www.ebi.ac.uk/pdbsum/5y31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5y31 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ADA22_HUMAN ADA22_HUMAN] Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1.<ref>PMID:12589811</ref> <ref>PMID:15882968</ref> <ref>PMID:16385342</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Epilepsy is a common brain disorder throughout history. Epilepsy-related ligand-receptor complex, LGI1-ADAM22, regulates synaptic transmission and has emerged as a determinant of brain excitability, as their mutations and acquired LGI1 autoantibodies cause epileptic disorders in human. Here, we report the crystal structure of human LGI1-ADAM22 complex, revealing a 2:2 heterotetrameric assembly. The hydrophobic pocket of the C-terminal epitempin-repeat (EPTP) domain of LGI1 binds to the metalloprotease-like domain of ADAM22. The N-terminal leucine-rich repeat and EPTP domains of LGI1 mediate the intermolecular LGI1-LGI1 interaction. A pathogenic R474Q mutation of LGI1, which does not exceptionally affect either the secretion or the ADAM22 binding, is located in the LGI1-LGI1 interface and disrupts the higher-order assembly of the LGI1-ADAM22 complex in vitro and in a mouse model for familial epilepsy. These studies support the notion that the LGI1-ADAM22 complex functions as the trans-synaptic machinery for precise synaptic transmission.
-Authors:
+Structural basis of epilepsy-related ligand-receptor complex LGI1-ADAM22.,Yamagata A, Miyazaki Y, Yokoi N, Shigematsu H, Sato Y, Goto-Ito S, Maeda A, Goto T, Sanbo M, Hirabayashi M, Shirouzu M, Fukata Y, Fukata M, Fukai S Nat Commun. 2018 Apr 18;9(1):1546. doi: 10.1038/s41467-018-03947-w. PMID:29670100<ref>PMID:29670100</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 5y31" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[A Disintegrin And Metalloproteinase 3D structures|A Disintegrin And Metalloproteinase 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Fukai S]]
+[[Category: Yamagata A]]