5y4s: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
m Protected "5y4s" [edit=sysop:move=sysop]
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
(3 intermediate revisions by the same user not shown)
Line 1: Line 1:
'''Unreleased structure'''


The entry 5y4s is ON HOLD
==Structure of a methyltransferase complex==
<StructureSection load='5y4s' size='340' side='right'caption='[[5y4s]], [[Resolution|resolution]] 3.41&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5y4s]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y4S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Y4S FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.405&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5y4s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y4s OCA], [https://pdbe.org/5y4s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5y4s RCSB], [https://www.ebi.ac.uk/pdbsum/5y4s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5y4s ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CHER1_PSEAE CHER1_PSEAE] Methylation of the membrane-bound methyl-accepting chemotaxis proteins (MCP) to form gamma-glutamyl methyl ester residues in MCP.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The bacterial second messenger cyclic di-GMP (c-di-GMP) has emerged as a prominent mediator of bacterial physiology, motility and pathogenicity. Cdi-GMP often regulates the function of its protein targets through a unique mechanism that involves a discrete PilZ adaptor protein. However, the molecular mechanism in c-di-GMP-mediated protein regulation is unclear. Here, we present the structure of the PilZ adaptor protein MapZ co-crystallized in complex with c-di-GMP and its protein target CheR1, a chemotaxis-regulating methyltransferase in Pseudomonas aeruginosa This co-crystal structure, together with the structure of free CheR1, revealed that the binding of c-di-GMP induces dramatic structural changes in MapZ that are crucial for CheR1 binding. Importantly, we found that restructuring and repositioning of two C-terminal helices enables MapZ to disrupt the CheR1 active site by dislodging a structural domain. The crystallographic observations are reinforced by proteinprotein binding and single cell-based flagellar motor switching analysis. Our studies further suggest that the regulation of chemotaxis by c-di-GMP through MapZ orthologs/homologs is widespread in proteobacteria, and that the use of allosterically regulated C-terminal motifs could be a common mechanism for PilZ adaptor proteins. Together, the findings provide detailed structural insights into how c-di-GMP controls the activity of an enzyme target indirectly through a PilZ adaptor protein.


Authors: Yan, X., Xin, L., Tan, Y.J., Jin, S., Liang, Z.X., Gao, Y.G.
Structural Analyses Unravel the Molecular Mechanism of Cyclic di-GMP Regulation of Bacterial Chemotaxis via a PilZ Adaptor Protein.,Yan XF, Xin L, Yen JT, Zeng Y, Jin S, Cheang QW, Fong RACY, Chiam KH, Liang ZX, Gao YG J Biol Chem. 2017 Nov 16. pii: M117.815704. doi: 10.1074/jbc.M117.815704. PMID:29146598<ref>PMID:29146598</ref>


Description: Structure of a methyltransferase complex
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Yan, X]]
<div class="pdbe-citations 5y4s" style="background-color:#fffaf0;"></div>
[[Category: Xin, L]]
 
[[Category: Jin, S]]
==See Also==
[[Category: Liang, Z.X]]
*[[Chemotaxis protein 3D structures|Chemotaxis protein 3D structures]]
[[Category: Tan, Y.J]]
== References ==
[[Category: Gao, Y.G]]
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Gao YG]]
[[Category: Jin S]]
[[Category: Liang ZX]]
[[Category: Tan YJ]]
[[Category: Xin L]]
[[Category: Yan X]]
Retrieved from "https://proteopedia.org/5y4s"