6el1: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: '''Unreleased structure''' The entry 6el1 is ON HOLD until Paper Publication Authors: Braeuning, B., Bertosin, E., Dietz, H., Groll, M. Description: YaxAB pore complex [[Category: Unre... |
No edit summary |
||
| (4 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==YaxAB pore complex== | |||
<SX load='6el1' size='340' side='right' viewer='molstar' caption='[[6el1]], [[Resolution|resolution]] 6.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6el1]] is a 20 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_enterocoliticum"_schleifstein_and_coleman_1939 "bacterium enterocoliticum" schleifstein and coleman 1939]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EL1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6EL1 FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6ek7|6ek7]], [[6ek8|6ek8]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ERS137951_00706 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=630 "Bacterium enterocoliticum" Schleifstein and Coleman 1939]), YE1985 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=630 "Bacterium enterocoliticum" Schleifstein and Coleman 1939])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6el1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6el1 OCA], [http://pdbe.org/6el1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6el1 RCSB], [http://www.ebi.ac.uk/pdbsum/6el1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6el1 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Pore-forming toxins (PFT) are virulence factors that transform from soluble to membrane-bound states. The Yersinia YaxAB system represents a family of binary alpha-PFTs with orthologues in human, insect, and plant pathogens, with unknown structures. YaxAB was shown to be cytotoxic and likely involved in pathogenesis, though the molecular basis for its two-component lytic mechanism remains elusive. Here, we present crystal structures of YaxA and YaxB, together with a cryo-electron microscopy map of the YaxAB complex. Our structures reveal a pore predominantly composed of decamers of YaxA-YaxB heterodimers. Both subunits bear membrane-active moieties, but only YaxA is capable of binding to membranes by itself. YaxB can subsequently be recruited to membrane-associated YaxA and induced to present its lytic transmembrane helices. Pore formation can progress by further oligomerization of YaxA-YaxB dimers. Our results allow for a comparison between pore assemblies belonging to the wider ClyA-like family of alpha-PFTs, highlighting diverse pore architectures. | |||
Structure and mechanism of the two-component alpha-helical pore-forming toxin YaxAB.,Brauning B, Bertosin E, Praetorius F, Ihling C, Schatt A, Adler A, Richter K, Sinz A, Dietz H, Groll M Nat Commun. 2018 May 4;9(1):1806. doi: 10.1038/s41467-018-04139-2. PMID:29728606<ref>PMID:29728606</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6el1" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Bacterium enterocoliticum schleifstein and coleman 1939]] | |||
[[Category: Large Structures]] | |||
[[Category: Bertosin, E]] | |||
[[Category: Braeuning, B]] | [[Category: Braeuning, B]] | ||
[[Category: Dietz, H]] | [[Category: Dietz, H]] | ||
[[Category: Groll, M]] | [[Category: Groll, M]] | ||
[[Category: Membrane protein]] | |||
[[Category: Pathogen]] | |||
[[Category: Pore-forming toxin]] | |||
[[Category: Two-component toxin]] | |||
Latest revision as of 00:27, 11 April 2020
YaxAB pore complex
| ||||||||
