5w94: Difference between revisions

Latest revision as of 12:17, 6 November 2024

Crystal structure of Scc4 in complex with Scc2n and Ctf19n

Structural highlights

5w94 is a 6 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.193Å
Ligands:	SEP, TPO
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SCC2_YEAST Plays a structural role in chromatin. Involved in sister chromatid cohesion. Forms a complex with SCC4 required for the stable association of the cohesin complex with chromatin, which may act by hydrolyzing ATP from SMC1 and SMC3 heads. Also participates in DNA repair.^[1] ^[2]

Publication Abstract from PubMed

The ring-shaped cohesin complex brings together distant DNA domains to maintain, express, and segregate the genome. Establishing specific chromosomal linkages depends on cohesin recruitment to defined loci. One such locus is the budding yeast centromere, which is a paradigm for targeted cohesin loading. The kinetochore, a multiprotein complex that connects centromeres to microtubules, drives the recruitment of high levels of cohesin to link sister chromatids together. We have exploited this system to determine the mechanism of specific cohesin recruitment. We show that phosphorylation of the Ctf19 kinetochore protein by a conserved kinase, DDK, provides a binding site for the Scc2/4 cohesin loading complex, thereby directing cohesin loading to centromeres. A similar mechanism targets cohesin to chromosomes in vertebrates. These findings represent a complete molecular description of targeted cohesin loading, a phenomenon with wide-ranging importance in chromosome segregation and, in multicellular organisms, transcription regulation.

The Kinetochore Receptor for the Cohesin Loading Complex.,Hinshaw SM, Makrantoni V, Harrison SC, Marston AL Cell. 2017 Sep 21;171(1):72-84.e13. doi: 10.1016/j.cell.2017.08.017. PMID:28938124^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ciosk R, Shirayama M, Shevchenko A, Tanaka T, Toth A, Shevchenko A, Nasmyth K. Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins. Mol Cell. 2000 Feb;5(2):243-54. PMID:10882066
↑ Arumugam P, Gruber S, Tanaka K, Haering CH, Mechtler K, Nasmyth K. ATP hydrolysis is required for cohesin's association with chromosomes. Curr Biol. 2003 Nov 11;13(22):1941-53. PMID:14614819
↑ Hinshaw SM, Makrantoni V, Harrison SC, Marston AL. The Kinetochore Receptor for the Cohesin Loading Complex. Cell. 2017 Sep 21;171(1):72-84.e13. doi: 10.1016/j.cell.2017.08.017. PMID:28938124 doi:https://dx.doi.org/10.1016/j.cell.2017.08.017

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OCA

[1] Ciosk R, Shirayama M, Shevchenko A, Tanaka T, Toth A, Shevchenko A, Nasmyth K. Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins. Mol Cell. 2000 Feb;5(2):243-54. PMID:10882066

[2] Arumugam P, Gruber S, Tanaka K, Haering CH, Mechtler K, Nasmyth K. ATP hydrolysis is required for cohesin's association with chromosomes. Curr Biol. 2003 Nov 11;13(22):1941-53. PMID:14614819

[3] Hinshaw SM, Makrantoni V, Harrison SC, Marston AL. The Kinetochore Receptor for the Cohesin Loading Complex. Cell. 2017 Sep 21;171(1):72-84.e13. doi: 10.1016/j.cell.2017.08.017. PMID:28938124 doi:https://dx.doi.org/10.1016/j.cell.2017.08.017

[1]

[2]

[3]

@@ Line 1: / Line 1: @@
 ==Crystal structure of Scc4 in complex with Scc2n and Ctf19n==
-<StructureSection load='5w94' size='340' side='right' caption='[[5w94]], [[Resolution|resolution]] 3.19&Aring;' scene=''>
+<StructureSection load='5w94' size='340' side='right'caption='[[5w94]], [[Resolution|resolution]] 3.19&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5w94]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W94 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5W94 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5w94]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5W94 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5W94 FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.193&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xdn|4xdn]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5w94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w94 OCA], [http://pdbe.org/5w94 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5w94 RCSB], [http://www.ebi.ac.uk/pdbsum/5w94 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5w94 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5w94 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5w94 OCA], [https://pdbe.org/5w94 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5w94 RCSB], [https://www.ebi.ac.uk/pdbsum/5w94 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5w94 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SCC4_YEAST SCC4_YEAST]] Involved in sister chromatid cohesion. Forms a complex with SCC2, which is required for the association of the cohesin complex with chromosomes.<ref>PMID:10882066</ref>  [[http://www.uniprot.org/uniprot/SCC2_YEAST SCC2_YEAST]] Plays a structural role in chromatin. Involved in sister chromatid cohesion. Forms a complex with SCC4 required for the stable association of the cohesin complex with chromatin, which may act by hydrolyzing ATP from SMC1 and SMC3 heads. Also participates in DNA repair.<ref>PMID:10882066</ref> <ref>PMID:14614819</ref>
+[https://www.uniprot.org/uniprot/SCC2_YEAST SCC2_YEAST] Plays a structural role in chromatin. Involved in sister chromatid cohesion. Forms a complex with SCC4 required for the stable association of the cohesin complex with chromatin, which may act by hydrolyzing ATP from SMC1 and SMC3 heads. Also participates in DNA repair.<ref>PMID:10882066</ref> <ref>PMID:14614819</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Harrison, S C]]
+[[Category: Large Structures]]
-[[Category: Hinshaw, S M]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: Cell cycle]]
+[[Category: Harrison SC]]
-[[Category: Centromere]]
+[[Category: Hinshaw SM]]
-[[Category: Cohesin]]
-[[Category: Kinetochore]]

5w94: Difference between revisions

Latest revision as of 12:17, 6 November 2024

Crystal structure of Scc4 in complex with Scc2n and Ctf19n

Structural highlights

Function

Publication Abstract from PubMed

References

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