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| <StructureSection load='4c2j' size='450' side='right' caption='Human 3-ketoacyl-CoA complex with CoA and ethylene glycol(PDB code [[4c2j]]).' scene='44/447919/Cv/1'> | | <StructureSection load='4c2j' size='350' side='right' caption='Human 3-ketoacyl-CoA complex with CoA and ethylene glycol (PDB code [[4c2j]])' scene='44/447919/Cv/7'> |
| == Function == | | == Function == |
| Two different classes of '''thiolases''' are found.<br /> | | Two different classes of '''thiolases''' are found.<br /> |
| *'''Degrative thiolase''' like '''3-ketoacyl-CoA thiolase''' (KCT) is involved in fatty acid β oxidation<ref>PMID:16630629</ref>.<br /> | | *'''Degrative thiolase''' like '''3-ketoacyl-CoA thiolase''' (KCT) is involved in fatty acid [[beta oxidation]]<ref>PMID:16630629</ref>.<br /> |
| *'''Biosynthetic thiolases''' like '''acetoacetyl-CoA thiolase''' (ACT) are involved in the thiolysis of acetoacetyl-CoA<ref>PMID:21908473</ref>. | | *'''Biosynthetic thiolases''' like '''acetoacetyl-CoA thiolase''' (ACT) or '''acetyl-CoA acetyltransferase''' are involved in the thiolysis of acetoacetyl-CoA<ref>PMID:21908473</ref>. |
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| For Bacterial thiolase see [[Bacterial thiolase]] | | For bacterial thiolase see [[Bacterial thiolase]] |
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| | *'''SCP2-thiolase''' converts in the bile acid synthesis pathway 24-keto-THC-CoA into choloyl-CoA and propionyl-CoA<ref>PMID:30573650</ref>. For zebrafish thiolase see [[SCP2-thiolase]]. ''' SCP''' is Sterol Carrier Protein. |
| | *'''U34-tRNA-thiolase''' thiolates uridine 34 in tRNA anticodon loop which guarantees the fidelity of protein translation in archaea<ref>PMID:37005440</ref>. |
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| | See also [[Mevalonate pathway]] |
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| == Structural highlights == | | == Structural highlights == |
| The <scene name='44/447919/Cv/2'>active site of 3-ketoacyl-CoA thiolase contains CoA</scene><ref>PMID:25478839</ref>. Water molecules shown as red spheres. | | The <scene name='44/447919/Cv/8'>active site of 3-ketoacyl-CoA thiolase contains CoA</scene><ref>PMID:25478839</ref>. Water molecules are shown as red spheres. |
| </StructureSection>
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| == 3D Structures of Thiolase == | | == 3D Structures of Thiolase == |
| | [[Thiolase 3D structures]] |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | </StructureSection> |
| {{#tree:id=OrganizedByTopic|openlevels=0|
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| *3-ketoacyl-CoA thiolase
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| **[[2wu9]], [[2c7y]], [[2c7z]] – KCT peroxisomal residues 36-462 – ''Arabidopsis thaliana''<br />
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| **[[2wua]] - KCT peroxisomal residues 17-449 (mutant) – sunflower<br />
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| **[[2iik]] – hKCT peroxisomal – human<br />
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| **[[4c2k]] – hKCT mitochondrial <br />
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| **[[4c2j]] – hKCT mitochondrial + CoA<br />
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| **[[3goa]] – KCT – ''Salmonella typhimurium''<br />
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| **[[5lp7]] – KCT – ''Bacillus subtilis'' <br />
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| **[[1afw]], [[1pxt]] – yKCT peroxisomal – yeast<br />
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| **[[5cbq]] – MsKCT – ''Mycobacterium smegmatis'' <br />
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| **[[4w61]] – ReKCT – ''Ralstonia eutropha''<br />
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| **[[4ubw]], [[5onc]] – MtKCT – ''Mycobacterium tuberculosis'' <br />
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| *3-ketoacyl-CoA thiolase complex
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| **[[3w15]] - yKCT peroxisomal residues 27-396 + peroxiomal membrane protein PEX21 + peroxisomal targeting signal receptor<br />
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| **[[2d3t]] – PfKCT + fatty oxidation complex β subunit – ''Pseudomonas fragi''<br />
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| **[[1wdk]], [[1wdl]], [[1wdm]] - PfKCT + fatty oxidation complex α subunit<br />
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| **[[5bz4]] – MsKCT + CoA <br />
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| **[[4ubt]] – MtKCT (mutant) + CoA + steroid <br />
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| **[[4ubu]] – MtKCT (mutant) + CoA <br />
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| **[[4ubv]] – MtKCT + CoA + acetyl CoA <br />
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| *Acetoacetyl-CoA thiolase
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| **[[1m4s]], [[1m4t]], [[1dlu]], [[1qfl]] - ZrACT – ''Zoogloea ramigera''<br />
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| **[[2wku]], [[2wl6]], [[1m1t]], [[1m3k]] - ZrACT (mutant) <br />
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| **[[2ib7]], [[2ib8]], [[2ib9]], [[2ibu]], [[2ibw]], [[2iby]], [[2f2s]] – hACT mitochondrial<br />
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| **[[1wl5]] - hACT cytosolic<br />
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| **[[4dd5]], [[4e1l]] – ACT – ''Clostridium difficile''<br />
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| **[[4xl2]], [[4xl3]], [[4n44]], [[4n45]] – CaACT – ''Clostridium acetobutylicum''<br />
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| **[[4wyr]] – CaACT (mutant) <br />
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| **[[5f0v]], [[5f38]], [[4wys]] – ACT – ''Escherichia coli'' <br />
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| **[[4o9a]], [[4o99]], [[4o9c]], [[4nzs]] – ReACT <br />
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| *Acetoacetyl-CoA thiolase complex
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| **[[1dm3]], [[1dlv]] – ZrACT + CoA<br />
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| **[[2wkt]], [[2wkv]], [[2wl4]], [[2wl5]], [[2vtz]], [[1m3z]] – ZrACT (mutant) + CoA<br />
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| **[[1m1o]] - ZrACT (mutant) + acetoacetyl-CoA<br />
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| **[[2vu0]], [[1nl7]] - ZrACT + CoA<br />
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| **[[2vu1]], [[2vu2]], [[1ou6]] - ZrACT + pantheteine-11-pivalate<br />
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| **[[1wl4]] – hACT cytosolic + CoA <br />
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| **[[4xl4]] – CaACT + CoA <br />
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| }}
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| == References == | | == References == |
| <references/> | | <references/> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |
