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==Crystal Structure of 4-Hydroxybutyryl-CoA Dehydratase from Clostridium aminobutyricum: Radical catalysis involving a [4Fe-4S] cluster and flavin==
==Crystal Structure of 4-Hydroxybutyryl-CoA Dehydratase from Clostridium aminobutyricum: Radical catalysis involving a [4Fe-4S] cluster and flavin==
<StructureSection load='1u8v' size='340' side='right' caption='[[1u8v]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
<StructureSection load='1u8v' size='340' side='right'caption='[[1u8v]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1u8v]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_aminobutyricum"_hardman_and_stadtman "clostridium aminobutyricum" hardman and stadtman]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U8V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U8V FirstGlance]. <br>
<table><tr><td colspan='2'>[[1u8v]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_aminobutyricum Clostridium aminobutyricum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U8V FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u8v OCA], [http://pdbe.org/1u8v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u8v RCSB], [http://www.ebi.ac.uk/pdbsum/1u8v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1u8v ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u8v OCA], [https://pdbe.org/1u8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u8v RCSB], [https://www.ebi.ac.uk/pdbsum/1u8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u8v ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/HDVD_CLOAM HDVD_CLOAM]] Catalyzes the reversible conversion of 4-hydroxybutyryl-CoA to crotonyl-CoA. The mechanism of the reaction seems to go through three steps: (1) the FAD-dependent oxidation of 4-hydroxybutyryl-CoA to 4-hydroxycrotonyl-CoA; (2) the hydroxyl group is substituted by a hydride derived from the now reduced FAD in an SN2' reaction leading to vinylacetyl-CoA; (3) isomerization to yield crotonyl-CoA.  
[https://www.uniprot.org/uniprot/HDVD_CLOAM HDVD_CLOAM] Catalyzes the reversible conversion of 4-hydroxybutyryl-CoA to crotonyl-CoA. The mechanism of the reaction seems to go through three steps: (1) the FAD-dependent oxidation of 4-hydroxybutyryl-CoA to 4-hydroxycrotonyl-CoA; (2) the hydroxyl group is substituted by a hydride derived from the now reduced FAD in an SN2' reaction leading to vinylacetyl-CoA; (3) isomerization to yield crotonyl-CoA.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u8/1u8v_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u8/1u8v_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
   </jmolCheckbox>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Clostridium aminobutyricum hardman and stadtman]]
[[Category: Clostridium aminobutyricum]]
[[Category: Buckel, W]]
[[Category: Large Structures]]
[[Category: Cinkaya, I]]
[[Category: Buckel W]]
[[Category: Dobbek, H]]
[[Category: Cinkaya I]]
[[Category: Martins, B M]]
[[Category: Dobbek H]]
[[Category: Messerschmidt, A]]
[[Category: Martins BM]]
[[Category: Alfa-helix]]
[[Category: Messerschmidt A]]
[[Category: Beta-strand]]
[[Category: Isomerase]]
[[Category: Lyase]]
Retrieved from "https://proteopedia.org/1u8v"