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[[Image:1ynt.gif|left|200px]]


{{Structure
==Structure of the monomeric form of T. gondii SAG1 surface antigen bound to a human Fab==
|PDB= 1ynt |SIZE=350|CAPTION= <scene name='initialview01'>1ynt</scene>, resolution 3.100&Aring;
<StructureSection load='1ynt' size='340' side='right'caption='[[1ynt]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>
<table><tr><td colspan='2'>[[1ynt]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Finegoldia_magna_ATCC_29328 Finegoldia magna ATCC 29328], [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YNT FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ynt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ynt OCA], [https://pdbe.org/1ynt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ynt RCSB], [https://www.ebi.ac.uk/pdbsum/1ynt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ynt ProSAT]</span></td></tr>
|RELATEDENTRY=[[1kzq|1KZQ]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ynt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ynt OCA], [http://www.ebi.ac.uk/pdbsum/1ynt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ynt RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/Q51918_FINMA Q51918_FINMA]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yn/1ynt_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ynt ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Toxoplasma gondii, the intracellular parasite responsible for toxoplasmosis infects more than one-third of the world population and can be life-threatening for fetuses and immunocompromised patients. The surface protein SAG1 is an important immune target, which provides a strong immune response against the invasive tachyzoite while the other forms of the parasite, devoid of SAG1 at their surface, are multiplying. In addition to this role as a "hot spot" decoy, SAG1 is predicted to act as an adhesin during host-cell attachment through its binding to proteoglycans. To begin to understand the relationships between SAG1 epitopes and the ligand-binding site, we have solved the crystal structure of the monomeric form of T.gondii SAG1 complexed to a Fab derived from a monoclonal antibody raised against tachyzoite particles. This antibody competes strongly with human Toxoplasma-specific sera, suggesting that its epitope is part of an immunodominant region present on the surface of SAG1. The structure reveals that this conformational epitope, located within the SAG1 N-terminal domain, does not overlap with the proposed ligand-binding pocket. This study provides the first structural description of the monomeric form of SAG1, and significant insights into its dual role of adhesin and immune target during parasite infection.


'''Structure of the immunodominant epitope displayed by the surface antigen 1 (SAG1) of Toxoplasma gondii complexed to a monoclonal antibody'''
Crystal structure of the complex between the monomeric form of Toxoplasma gondii surface antigen 1 (SAG1) and a monoclonal antibody that mimics the human immune response.,Graille M, Stura EA, Bossus M, Muller BH, Letourneur O, Battail-Poirot N, Sibai G, Gauthier M, Rolland D, Le Du MH, Ducancel F J Mol Biol. 2005 Nov 25;354(2):447-58. Epub 2005 Sep 30. PMID:16242717<ref>PMID:16242717</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1ynt" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
Toxoplasma gondii, the intracellular parasite responsible for toxoplasmosis infects more than one-third of the world population and can be life-threatening for fetuses and immunocompromised patients. The surface protein SAG1 is an important immune target, which provides a strong immune response against the invasive tachyzoite while the other forms of the parasite, devoid of SAG1 at their surface, are multiplying. In addition to this role as a "hot spot" decoy, SAG1 is predicted to act as an adhesin during host-cell attachment through its binding to proteoglycans. To begin to understand the relationships between SAG1 epitopes and the ligand-binding site, we have solved the crystal structure of the monomeric form of T.gondii SAG1 complexed to a Fab derived from a monoclonal antibody raised against tachyzoite particles. This antibody competes strongly with human Toxoplasma-specific sera, suggesting that its epitope is part of an immunodominant region present on the surface of SAG1. The structure reveals that this conformational epitope, located within the SAG1 N-terminal domain, does not overlap with the proposed ligand-binding pocket. This study provides the first structural description of the monomeric form of SAG1, and significant insights into its dual role of adhesin and immune target during parasite infection.
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
 
== References ==
==About this Structure==
<references/>
1YNT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna], [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YNT OCA].
__TOC__
 
</StructureSection>
==Reference==
[[Category: Finegoldia magna ATCC 29328]]
Crystal structure of the complex between the monomeric form of Toxoplasma gondii surface antigen 1 (SAG1) and a monoclonal antibody that mimics the human immune response., Graille M, Stura EA, Bossus M, Muller BH, Letourneur O, Battail-Poirot N, Sibai G, Gauthier M, Rolland D, Le Du MH, Ducancel F, J Mol Biol. 2005 Nov 25;354(2):447-58. Epub 2005 Sep 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16242717 16242717]
[[Category: Large Structures]]
[[Category: Finegoldia magna]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Toxoplasma gondii]]
[[Category: Toxoplasma gondii]]
[[Category: Battail-Poirot, N]]
[[Category: Battail-Poirot N]]
[[Category: Bossus, M.]]
[[Category: Bossus M]]
[[Category: Du, M H.Le.]]
[[Category: Ducancel F]]
[[Category: Ducancel, F.]]
[[Category: Graille M]]
[[Category: Graille, M.]]
[[Category: Le Du MH]]
[[Category: Letourneur, O.]]
[[Category: Letourneur O]]
[[Category: Muller, B H.]]
[[Category: Muller BH]]
[[Category: Rolland, D.]]
[[Category: Rolland D]]
[[Category: Sibai, G.]]
[[Category: Sibai G]]
[[Category: Stura, E A.]]
[[Category: Stura EA]]
[[Category: conformational epitope]]
[[Category: crystal structure]]
[[Category: recombinant sag1]]
[[Category: toxoplasma gondii]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:14:50 2008''
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