1zga: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
 
(14 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1zga.gif|left|200px]]


{{Structure
==Crystal structure of isoflavanone 4'-O-methyltransferase complexed with (+)-6a-hydroxymaackiain==
|PDB= 1zga |SIZE=350|CAPTION= <scene name='initialview01'>1zga</scene>, resolution 2.35&Aring;
<StructureSection load='1zga' size='340' side='right'caption='[[1zga]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=HMK:(6AR,12AR)-6H-[1,3]DIOXOLO[5,6][1]BENZOFURO[3,2-C]CHROMENE-3,6A(12AH)-DIOL'>HMK</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>
<table><tr><td colspan='2'>[[1zga]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZGA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZGA FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HMK:(6AR,12AR)-6H-[1,3]DIOXOLO[5,6][1]BENZOFURO[3,2-C]CHROMENE-3,6A(12AH)-DIOL'>HMK</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zga OCA], [https://pdbe.org/1zga PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zga RCSB], [https://www.ebi.ac.uk/pdbsum/1zga PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zga ProSAT]</span></td></tr>
|RELATEDENTRY=[[1zhf|1ZHF]], [[1zg3|1ZG3]], [[1zgj|1ZGJ]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zga OCA], [http://www.ebi.ac.uk/pdbsum/1zga PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zga RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/I4OMT_MEDTR I4OMT_MEDTR]
 
== Evolutionary Conservation ==
'''Crystal structure of isoflavanone 4'-O-methyltransferase complexed with (+)-6a-hydroxymaackiain'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zg/1zga_consurf.spt"</scriptWhenChecked>
In leguminous plants such as pea (Pisum sativum), alfalfa (Medicago sativa), barrel medic (Medicago truncatula), and chickpea (Cicer arietinum), 4'-O-methylation of isoflavonoid natural products occurs early in the biosynthesis of defense chemicals known as phytoalexins. However, among these four species, only pea catalyzes 3-O-methylation that converts the pterocarpanoid isoflavonoid 6a-hydroxymaackiain to pisatin. In pea, pisatin is important for chemical resistance to the pathogenic fungus Nectria hematococca. While barrel medic does not biosynthesize 6a-hydroxymaackiain, when cell suspension cultures are fed 6a-hydroxymaackiain, they accumulate pisatin. In vitro, hydroxyisoflavanone 4'-O-methyltransferase (HI4'OMT) from barrel medic exhibits nearly identical steady state kinetic parameters for the 4'-O-methylation of the isoflavonoid intermediate 2,7,4'-trihydroxyisoflavanone and for the 3-O-methylation of the 6a-hydroxymaackiain isoflavonoid-derived pterocarpanoid intermediate found in pea. Protein x-ray crystal structures of HI4'OMT substrate complexes revealed identically bound conformations for the 2S,3R-stereoisomer of 2,7,4'-trihydroxyisoflavanone and the 6aR,11aR-stereoisomer of 6a-hydroxymaackiain. These results suggest how similar conformations intrinsic to seemingly distinct chemical substrates allowed leguminous plants to use homologous enzymes for two different biosynthetic reactions. The three-dimensional similarity of natural small molecules represents one explanation for how plants may rapidly recruit enzymes for new biosynthetic reactions in response to changing physiological and ecological pressures.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1ZGA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZGA OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zga ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
Structural basis for dual functionality of isoflavonoid O-methyltransferases in the evolution of plant defense responses., Liu CJ, Deavours BE, Richard SB, Ferrer JL, Blount JW, Huhman D, Dixon RA, Noel JP, Plant Cell. 2006 Dec;18(12):3656-69. Epub 2006 Dec 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17172354 17172354]
</StructureSection>
[[Category: Large Structures]]
[[Category: Medicago truncatula]]
[[Category: Medicago truncatula]]
[[Category: Single protein]]
[[Category: Deavours BE]]
[[Category: Deavours, B E.]]
[[Category: Dixon RA]]
[[Category: Dixon, R A.]]
[[Category: Ferrer J-L]]
[[Category: Ferrer, J L.]]
[[Category: Liu C-J]]
[[Category: Liu, C J.]]
[[Category: Noel JP]]
[[Category: Noel, J P.]]
[[Category: Richard S]]
[[Category: Richard, S.]]
[[Category: isoflavanone 4'-o-methyltransferase]]
[[Category: rossman fold]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:35:39 2008''
Retrieved from "https://proteopedia.org/1zga"