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| <StructureSection load='1eqq' size='450' side='right' scene='' caption='E. coli SSB complex with ssDNA (stick model) and UMP [[1eqq]]'> | | <StructureSection load='1eqq' size='350' side='right' scene='' caption='E. coli SSB complex with ssDNA (stick model) and UMP [[1eqq]]'> |
| '''Single-stranded DNA-binding protein''' (SSB) binds to single-stranded regions of DNA (ssDNA) to protect it from annealing, prevention of secondary structure formation and digestion. SSB binds to DNA in a sequence-independent manner. The binding of SSB enables processes like replication, DNA repair and recombination to occur. SSB can form complexes with specific genome maintenance proteins helping their activities<ref>PMID:22976174</ref>.<br /> | | '''Single-stranded DNA-binding protein''' (SSB) binds to single-stranded regions of DNA (ssDNA) to protect it from annealing, prevention of secondary structure formation and digestion. SSB binds to DNA in a sequence-independent manner. The binding of SSB enables processes like replication, DNA repair and recombination to occur. SSB can form complexes with specific genome maintenance proteins helping their activities<ref>PMID:22976174</ref>.<br /> |
| * '''Viral SSB proteins''' are monomeric.<br /> | | * '''Viral SSB proteins''' are monomeric.<br /> |
| * '''Eukaryotic SSB''' ('''Replication protein A''' – RPA) is a heterotrimer composed of RPA70, RPA32 and RPA14. RPA has 6 DNA-binding domains (DBD).<br /> | | * '''Eukaryotic SSB''' ('''Replication protein A''' – RPA) is a heterotrimer composed of RPA70, RPA32 and RPA14. RPA has 6 DNA-binding domains (DBD).<br /> |
| | *'''Replication protein A70''' is the eukaryotic SSB 70kDa subunit<ref>PMID:11470540</ref>. |
| * '''Mitochondrial human SSB (HsmtSSB)''' is a homotetramer. | | * '''Mitochondrial human SSB (HsmtSSB)''' is a homotetramer. |
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| See also [[Single stranded binding protein]]. | | See also [[Single stranded binding protein]]. |
| </StructureSection>
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| == 3D Structures of single-stranded DNA-binding protein == | | == 3D Structures of single-stranded DNA-binding protein == |
| | [[Single-stranded DNA-binding protein 3D structures]] |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | </StructureSection> |
| {{#tree:id=OrganizedByTopic|openlevels=0|
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| *Single-stranded DNA-binding protein
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| **[[1gpc]] – SSB gp32 – Enterobacteria phage T4 <br />
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| **[[2a1k]] – SSB gp32 – Enterobacteria phage RB69 <br />
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| **[[1je5]] – SSB – Enterobacteria phage T7 <br />
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| **[[1vqe]], [[1yha]], [[1vqb]] – F1SSB – Enterobacteria phage F1<br />
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| **[[1vqa]], [[1vqc]], [[1vqd]], [[1vqe]], [[1vqf]], [[1vqg]], [[1vqh]], [[1vqi]], [[1vqj]], [[1ae2]], [[1ae3]], [[1gkh]] – F1SSB (mutant) <br />
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| **[[2gn5]] – M13SSB – Enterobacteria phage M13<br />
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| **[[2gva]], [[2gvb]] – M13SSB (mutant) - NMR<br />
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| **[[1adu]], [[1adv]], [[1anv]], [[2waz]], [[2wb0]] – SSB C terminal – human adenovirus 5 <br />
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| **[[1urj]] – SSB – human herpesvirus 1 <br />
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| **[[1pfs]] – SSB (mutant) – Pseudomonas phage PF3 - NMR<br />
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| **[[3ull]], [[1s3o]], [[2dud]] – HsmtSSB residues 16-148 – human <br />
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| **[[1kaw]] – EcSSB chymotryptic domain – ''Escherichia coli'' <br />
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| **[[1qvc]], [[1sru]] – EcSSB <br />
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| **[[1o7i]] – SsSSB residues 1-119 – ''Sulfolobus solfataricus''<br />
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| **[[1ue1]], [[1ue5]], [[1ue7]], [[1ue6]] – SSB – ''Mycobacterium tuberculosis'' <br />
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| **[[1x3e]], [[1x3f]], [[1x3g]], [[5gqo]] – MsSSB – ''Mycobacterium smegmatis'' <br />
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| **[[1se8]] – DrSSB – ''Deinococcus radiodurans'' <br />
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| **[[4exw]] – SSB DdrB – ''Deinococcus geothermalis'' <br />
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| **[[1z9f]] – SSB – ''Thermotoga maritima'' <br />
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| **[[2cwa]] – SSB – ''Thermus thermophilus'' <br />
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| **[[2fxq]], [[2ihe]] – TaSSB – ''Thermus aquaticus'' <br />
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| **[[2ihf]] – TaSSB (mutant) <br />
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| **[[2hql]] – SSB – ''Mycoplasma pneumoniae'' <br />
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| **[[3eiv]] – ScSSB – ''Streptomyces coelicolor''<br />
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| **[[4dam]] – ScSSB (mutant) <br />
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| **[[3lgj]], [[3pgz]] – SSB – ''Bartonella henselae'' <br />
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| **[[3afp]], [[3afq]] – SSB – ''Mycobacterium leprae'' <br />
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| **[[3tqy]] – SSB – ''Coxiella burnetii'' <br />
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| **[[3tek]] – SSB N terminal – ''Thermoproteus tenax''<br />
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| **[[4gs3]] – SSB N terminal – ''Thermoanaerobacter tengcongensis''<br />
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| **[[3dm3]] – SSB A domain – ''Methancaldococcus jannaschii''<br />
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| **[[5xgt]] – SSB N-terminal – ''Staphylococcus aureus''<br />
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| **[[5m1x]] – ySSB N-OB domain (mutant) - yeast<br />
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| **[[5d8e]], [[5d8f]] – hSSB residues 1-109 (mutant) - human<br />
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| *Single-stranded DNA-binding protein complex with ssDNA
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| **[[1eyg]] – EcSSB chymotryptic domain + ssDNA <br />
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| **[[1eqq]] – EcSSB + ssDNA <br />
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| **[[2vw9]] – HpSSB + ssDNA <br />
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| **[[3vdy]] – SSB + ssDNA – ''Bacillus subtilis''<br />
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| **[[3a5u]] – MsSSB chymotryptic domain + ssDNA <br />
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| **[[3udg]] – DrSSB + ssDNA <br />
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| **[[2mna]] – SsSSB + ssDNA - NMR<br />
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| *Other single-stranded DNA-binding protein complexes
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| **[[3ufm]], [[3uf7]] – DrSSB C terminal + uracil-DNA glycosylase <br />
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| *Replication protein A70
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| **[[1ewi]], [[2b29]] - hRPA70 N terminal - NMR<br />
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| **[[4ipc]], [[4ipd]], [[4ipg]] - hRPA70 N terminal (mutant) <br />
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| **[[1fgu]] – hRPA70 central domain <br />
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| **[[1ynx]] – yRPA69 DBD-A – NMR<br />
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| **[[1jmc]] – hRPA70 central domain + DNA <br />
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| **[[2b3g]] - hRPA70 N terminal + p53 peptide<br />
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| **[[5eay]] - hRPA70 N terminal + DNA2 peptide<br />
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| **[[4nb3]], [[5n85]] - hRPA70 N terminal (mutant) + peptide<br />
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| **[[5n8a]] - hRPA70 N terminal (mutant) + PrimPol<br />
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| **[[4ijh]], [[4ijl]], [[4iph]], [[4luo]], [[4luv]], [[4luz]], [[4lw1]], [[4lwc]], [[4o0a]], [[4r4c]], [[4r4i]], [[4r4o]], [[4r4q]], [[4r4t]], [[5e7n]] - hRPA70 N terminal (mutant) + inhibitor<br />
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| *Replication protein A70+A14+A32
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| **[[1l1o]] – hRPA14 + hRPA32 central domain + hRPA70 C terminal <br />
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| }}
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| == References == | | == References == |
| <references/> | | <references/> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |
