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| ==THE CRYSTAL STRUCTURE OF THE SOLUBLE DOMAIN OF SULFOLOBUS ACIDOCALDARIUS FLAF== | | ==THE CRYSTAL STRUCTURE OF THE SOLUBLE DOMAIN OF SULFOLOBUS ACIDOCALDARIUS FLAF== |
| <StructureSection load='4zbh' size='340' side='right' caption='[[4zbh]], [[Resolution|resolution]] 1.50Å' scene=''> | | <StructureSection load='4zbh' size='340' side='right'caption='[[4zbh]], [[Resolution|resolution]] 1.50Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[4zbh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulac Sulac]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZBH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZBH FirstGlance]. <br> | | <table><tr><td colspan='2'>[[4zbh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius_DSM_639 Sulfolobus acidocaldarius DSM 639]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZBH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZBH FirstGlance]. <br> |
| </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Saci_1175 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=330779 SULAC])</td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5Å</td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zbh OCA], [http://pdbe.org/4zbh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zbh RCSB], [http://www.ebi.ac.uk/pdbsum/4zbh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zbh ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zbh OCA], [https://pdbe.org/4zbh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zbh RCSB], [https://www.ebi.ac.uk/pdbsum/4zbh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zbh ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/Q4J9K8_SULAC Q4J9K8_SULAC] |
| Archaea employ the archaellum, a type IV pilus-like nanomachine, for swimming motility. In the crenarchaeon Sulfolobus acidocaldarius, the archaellum consists of seven proteins: FlaB/X/G/F/H/I/J. FlaF is conserved and essential for archaellum assembly but no FlaF structures exist. Here, we truncated the FlaF N terminus and solved 1.5-A and 1.65-A resolution crystal structures of this monotopic membrane protein. Structures revealed an N-terminal alpha-helix and an eight-strand beta-sandwich, immunoglobulin-like fold with striking similarity to S-layer proteins. Crystal structures, X-ray scattering, and mutational analyses suggest dimer assembly is needed for in vivo function. The sole cell envelope component of S. acidocaldarius is a paracrystalline S-layer, and FlaF specifically bound to S-layer protein, suggesting that its interaction domain is located in the pseudoperiplasm with its N-terminal helix in the membrane. From these data, FlaF may act as the previously unknown archaellum stator protein that anchors the rotating archaellum to the archaeal cell envelope.
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| FlaF Is a beta-Sandwich Protein that Anchors the Archaellum in the Archaeal Cell Envelope by Binding the S-Layer Protein.,Banerjee A, Tsai CL, Chaudhury P, Tripp P, Arvai AS, Ishida JP, Tainer JA, Albers SV Structure. 2015 Apr 3. pii: S0969-2126(15)00079-9. doi:, 10.1016/j.str.2015.03.001. PMID:25865246<ref>PMID:25865246</ref>
| | ==See Also== |
| | | *[[Flagellar protein 3D structures|Flagellar protein 3D structures]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 4zbh" style="background-color:#fffaf0;"></div>
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| == References == | |
| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Sulac]] | | [[Category: Large Structures]] |
| [[Category: Arvai, A S]] | | [[Category: Sulfolobus acidocaldarius DSM 639]] |
| [[Category: Ishida, J P]] | | [[Category: Arvai AS]] |
| [[Category: Tainer, J A]] | | [[Category: Ishida JP]] |
| [[Category: Tsai, C L]] | | [[Category: Tainer JA]] |
| [[Category: Immune system]] | | [[Category: Tsai C-L]] |
| [[Category: Immunoglobulin-like beta-sandwich]]
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| [[Category: Stator protein]]
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