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==Crystal structure of the N-terminal DCB-HUS domain of T. terrestris Sec7==
==Crystal structure of the N-terminal DCB-HUS domain of T. terrestris Sec7==
<StructureSection load='5has' size='340' side='right' caption='[[5has]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
<StructureSection load='5has' size='340' side='right'caption='[[5has]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5has]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Acremonium_alabamense Acremonium alabamense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HAS FirstGlance]. <br>
<table><tr><td colspan='2'>[[5has]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermothielavioides_terrestris Thermothielavioides terrestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HAS FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Sec7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=35720 Acremonium alabamense])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.653&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5has FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5has OCA], [http://pdbe.org/5has PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5has RCSB], [http://www.ebi.ac.uk/pdbsum/5has PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5has ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5has FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5has OCA], [https://pdbe.org/5has PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5has RCSB], [https://www.ebi.ac.uk/pdbsum/5has PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5has ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A0A140UHP8_9PEZI A0A140UHP8_9PEZI]
The Golgi complex is the central sorting compartment of eukaryotic cells. Arf guanine nucleotide exchange factors (Arf-GEFs) regulate virtually all traffic through the Golgi by activating Arf GTPase trafficking pathways. The Golgi Arf-GEFs contain multiple autoregulatory domains, but the precise mechanisms underlying their function remain largely undefined. We report a crystal structure revealing that the N-terminal DCB and HUS regulatory domains of the Arf-GEF Sec7 form a single structural unit. We demonstrate that the established role of the N-terminal region in dimerization is not conserved; instead, a C-terminal autoinhibitory domain is responsible for dimerization of Sec7. We find that the DCB/HUS domain amplifies the ability of Sec7 to activate Arf1 on the membrane surface by facilitating membrane insertion of the Arf1 amphipathic helix. This enhancing function of the Sec7 N-terminal domains is consistent with the high rate of Arf1-dependent trafficking to the plasma membrane necessary for maximal cell growth.
 
The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase.,Richardson BC, Halaby SL, Gustafson MA, Fromme JC Elife. 2016 Jan 14;5. pii: e12411. doi: 10.7554/eLife.12411. PMID:26765562<ref>PMID:26765562</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5has" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Acremonium alabamense]]
[[Category: Large Structures]]
[[Category: Fromme, J C]]
[[Category: Thermothielavioides terrestris]]
[[Category: Richardson, B C]]
[[Category: Fromme JC]]
[[Category: Arf-gef]]
[[Category: Richardson BC]]
[[Category: Armadillo]]
[[Category: Protein transport]]
[[Category: Tgn]]
[[Category: Transport]]
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