|
|
| (11 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
| [[Image:264d.jpg|left|200px]]
| |
|
| |
|
| {{Structure
| | ==THREE-DIMENSIONAL CRYSTAL STRUCTURE OF THE A-TRACT DNA DODECAMER D(CGCAAATTTGCG) COMPLEXED WITH THE MINOR-GROOVE-BINDING DRUG HOECHST 33258== |
| |PDB= 264d |SIZE=350|CAPTION= <scene name='initialview01'>264d</scene>, resolution 2.440Å
| | <StructureSection load='264d' size='340' side='right'caption='[[264d]], [[Resolution|resolution]] 2.44Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=HT:2'-(4-HYDROXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5'-BI-BENZIMIDAZOLE'>HT</scene>
| | <table><tr><td colspan='2'>[[264d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=264D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=264D FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.44Å</td></tr> |
| |GENE=
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HT:2-(4-HYDROXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5-BI-BENZIMIDAZOLE'>HT</scene></td></tr> |
| |DOMAIN=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=264d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=264d OCA], [https://pdbe.org/264d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=264d RCSB], [https://www.ebi.ac.uk/pdbsum/264d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=264d ProSAT]</span></td></tr> |
| |RELATEDENTRY=
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=264d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=264d OCA], [http://www.ebi.ac.uk/pdbsum/264d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=264d RCSB]</span>
| | __TOC__ |
| }}
| | </StructureSection> |
| | | [[Category: Large Structures]] |
| '''THREE-DIMENSIONAL CRYSTAL STRUCTURE OF THE A-TRACT DNA DODECAMER D(CGCAAATTTGCG) COMPLEXED WITH THE MINOR-GROOVE-BINDING DRUG HOECHST 33258'''
| | [[Category: Aymami J]] |
| | | [[Category: Coll M]] |
| | | [[Category: Eritja R]] |
| ==Overview==
| | [[Category: Garcia-Saez I]] |
| The molecular structure of the DNA A-tract dodecamer d(CGCAAATTTGCG) complexed with the drug Hoechst 33258 has been determined by X-ray diffraction analysis. The Hoechst molecule binds in the DNA minor groove covering the sequence AATTT of the central A-tract, with the piperazine group close to one of the GC regions. The drug molecule makes two three-centered hydrogen bonds from the nitrogen atoms of the benzimidazole rings to the N3 and O2 atoms of the DNA bases. Although a high propeller twist is observed in the A-tract, only one unsymmetrical three-centered hydrogen bond is present in the DNA major groove. The structure is compared with other minor-groove-binding drug complexes and the influence of these drugs on DNA A-tracts is discussed.
| | [[Category: Rich A]] |
| | | [[Category: Van Boom JH]] |
| ==About this Structure==
| | [[Category: Van Der Marel GA]] |
| 264D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=264D OCA].
| | [[Category: Vega MC]] |
| | |
| ==Reference==
| |
| Three-dimensional crystal structure of the A-tract DNA dodecamer d(CGCAAATTTGCG) complexed with the minor-groove-binding drug Hoechst 33258., Vega MC, Garcia Saez I, Aymami J, Eritja R, Van der Marel GA, Van Boom JH, Rich A, Coll M, Eur J Biochem. 1994 Jun 15;222(3):721-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7517864 7517864]
| |
| [[Category: Protein complex]] | |
| [[Category: Aymami, J.]] | |
| [[Category: Boom, J H.Van.]]
| |
| [[Category: Coll, M.]] | |
| [[Category: Eritja, R.]] | |
| [[Category: Garcia-Saez, I.]] | |
| [[Category: Marel, G A.Van Der.]]
| |
| [[Category: Rich, A.]] | |
| [[Category: Vega, M C.]] | |
| [[Category: b-dna]] | |
| [[Category: complexed with drug]] | |
| [[Category: double helix]]
| |
| | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:45:36 2008''
| |
