|
|
| (11 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
| [[Image:283d.jpg|left|200px]]
| |
|
| |
|
| {{Structure
| | ==A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING== |
| |PDB= 283d |SIZE=350|CAPTION= <scene name='initialview01'>283d</scene>, resolution 2.300Å
| | <StructureSection load='283d' size='340' side='right'caption='[[283d]], [[Resolution|resolution]] 2.30Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
| | <table><tr><td colspan='2'>[[283d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=283D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=283D FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| |GENE=
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> |
| |DOMAIN=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=283d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=283d OCA], [https://pdbe.org/283d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=283d RCSB], [https://www.ebi.ac.uk/pdbsum/283d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=283d ProSAT]</span></td></tr> |
| |RELATEDENTRY=
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=283d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=283d OCA], [http://www.ebi.ac.uk/pdbsum/283d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=283d RCSB]</span>
| | __TOC__ |
| }}
| | </StructureSection> |
| | | [[Category: Large Structures]] |
| '''A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING'''
| | [[Category: Baeyens KJ]] |
| | | [[Category: De Bondt HL]] |
| | | [[Category: Holbrook SR]] |
| ==Overview==
| | [[Category: Pardi A]] |
| The crystal structure of the RNA dodecamer 5'-GGCC(GAAA)GGCC-3' has been determined from x-ray diffraction data to 2.3-A resolution. In the crystal, these oligomers form double helices around twofold symmetry axes. Four consecutive non-Watson-Crick base pairs make up an internal loop in the middle of the duplex, including sheared G.A pairs and novel asymmetric A.A pairs. This internal loop sequence produces a significant curvature and narrowing of the double helix. The helix is curved by 34 degrees from end to end and the diameter is narrowed by 24% in the internal loop. A Mn2+ ion is bound directly to the N7 of the first guanine in the Watson-Crick region following the internal loop and the phosphate of the preceding residue. This Mn2+ location corresponds to a metal binding site observed in the hammerhead catalytic RNA.
| |
| | |
| ==About this Structure==
| |
| 283D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=283D OCA].
| |
| | |
| ==Reference==
| |
| A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing., Baeyens KJ, De Bondt HL, Pardi A, Holbrook SR, Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12851-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8917508 8917508]
| |
| [[Category: ]]
| |
| [[Category: Protein complex]] | |
| [[Category: Baeyens, K J.]] | |
| [[Category: Bondt, H L.De.]] | |
| [[Category: Holbrook, S R.]] | |
| [[Category: Pardi, A.]] | |
| [[Category: curved]]
| |
| [[Category: double helix]]
| |
| [[Category: internal loop]]
| |
| [[Category: mismatched]]
| |
| [[Category: unusual rna]]
| |
| | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:45:52 2008''
| |
