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==The neutron crystallographic structure of inorganic pyrophosphatase from Thermococcus thioreducens==
==The neutron crystallographic structure of inorganic pyrophosphatase from Thermococcus thioreducens==
<StructureSection load='3q3l' size='340' side='right' caption='[[3q3l]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='3q3l' size='340' side='right'caption='[[3q3l]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3q3l]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_baa-394 Atcc baa-394]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q3L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3Q3L FirstGlance]. <br>
<table><tr><td colspan='2'>[[3q3l]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_thioreducens Thermococcus thioreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q3L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q3L FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DOD:DEUTERATED+WATER'>DOD</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Neutron Diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3i98|3i98]], [[3q46|3q46]], [[3q4w|3q4w]], [[3q5v|3q5v]], [[3q9m|3q9m]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DOD:DEUTERATED+WATER'>DOD</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tt-IPPase ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=277988 ATCC BAA-394])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q3l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q3l OCA], [https://pdbe.org/3q3l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q3l RCSB], [https://www.ebi.ac.uk/pdbsum/3q3l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q3l ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3q3l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q3l OCA], [http://pdbe.org/3q3l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3q3l RCSB], [http://www.ebi.ac.uk/pdbsum/3q3l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3q3l ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/H2L2L6_9EURY H2L2L6_9EURY] Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.[HAMAP-Rule:MF_00209]
Inorganic pyrophosphatase (IPPase) from the archaeon Thermococcus thioreducens was cloned, overexpressed in Escherichia coli, purified and crystallized in restricted geometry, resulting in large crystal volumes exceeding 5 mm3. IPPase is thermally stable and is able to resist denaturation at temperatures above 348 K. Owing to the high temperature tolerance of the enzyme, the protein was amenable to room-temperature manipulation at the level of protein preparation, crystallization and X-ray and neutron diffraction analyses. A complete synchrotron X-ray diffraction data set to 1.85 A resolution was collected at room temperature from a single crystal of IPPase (monoclinic space group C2, unit-cell parameters a=106.11, b=95.46, c=113.68 A, alpha=gamma=90.0, beta=98.12 degrees ). As large-volume crystals of IPPase can be obtained, preliminary neutron diffraction tests were undertaken. Consequently, Laue diffraction images were obtained, with reflections observed to 2.1 A resolution with I/sigma(I) greater than 2.5. The preliminary crystallographic results reported here set in place future structure-function and mechanism studies of IPPase.


Inorganic pyrophosphatase crystals from Thermococcus thioreducens for X-ray and neutron diffraction.,Hughes RC, Coates L, Blakeley MP, Tomanicek SJ, Langan P, Kovalevsky AY, Garcia-Ruiz JM, Ng JD Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1482-7., doi: 10.1107/S1744309112032447. Epub 2012 Nov 14. PMID:23192028<ref>PMID:23192028</ref>
==See Also==
 
*[[Inorganic pyrophosphatase 3D structures|Inorganic pyrophosphatase 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3q3l" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc baa-394]]
[[Category: Large Structures]]
[[Category: Blakeley, M P]]
[[Category: Thermococcus thioreducens]]
[[Category: Coates, L]]
[[Category: Blakeley MP]]
[[Category: Garcia-Ruiz, J M]]
[[Category: Coates L]]
[[Category: Hughes, R C]]
[[Category: Garcia-Ruiz JM]]
[[Category: Meehan, E J]]
[[Category: Hughes RC]]
[[Category: Ng, J D]]
[[Category: Meehan EJ]]
[[Category: Tomanicek, S J]]
[[Category: Ng JD]]
[[Category: Hydrolase]]
[[Category: Tomanicek SJ]]
[[Category: Inorganic pyrophosphatase]]
[[Category: Neutron]]
[[Category: Phosphatase]]
[[Category: Pyrophosphatase]]
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