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[[Image:2acv.gif|left|200px]]


{{Structure
==Crystal Structure of Medicago truncatula UGT71G1==
|PDB= 2acv |SIZE=350|CAPTION= <scene name='initialview01'>2acv</scene>, resolution 2.&Aring;
<StructureSection load='2acv' size='340' side='right'caption='[[2acv]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=UDP:URIDINE-5&#39;-DIPHOSPHATE'>UDP</scene>
<table><tr><td colspan='2'>[[2acv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ACV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ACV FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
|GENE= UGT71G1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3880 Medicago truncatula])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2acv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2acv OCA], [https://pdbe.org/2acv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2acv RCSB], [https://www.ebi.ac.uk/pdbsum/2acv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2acv ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2acv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2acv OCA], [http://www.ebi.ac.uk/pdbsum/2acv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2acv RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/Q5IFH7_MEDTR Q5IFH7_MEDTR]
 
== Evolutionary Conservation ==
'''Crystal Structure of Medicago truncatula UGT71G1'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ac/2acv_consurf.spt"</scriptWhenChecked>
Glycosylation is a ubiquitous reaction controlling the bioactivity and storage of plant natural products. Glycosylation of small molecules is catalyzed by a superfamily of glycosyltransferases (GTs) in most plant species studied to date. We present crystal structures of the UDP flavonoid/triterpene GT UGT71G1 from Medicago truncatula bound to UDP or UDP-glucose. The structures reveal the key residues involved in the recognition of donor substrate and, by comparison with other GT structures, suggest His-22 as the catalytic base and Asp-121 as a key residue that may assist deprotonation of the acceptor by forming an electron transfer chain with the catalytic base. Mutagenesis confirmed the roles of these key residues in donor substrate binding and enzyme activity. Our results provide an initial structural basis for understanding the complex substrate specificity and regiospecificity underlying the glycosylation of plant natural products and other small molecules. This information will direct future attempts to engineer bioactive compounds in crop plants to improve plant, animal, and human health and to facilitate the rational design of GTs to improve the storage and stability of novel engineered bioactive compounds.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
2ACV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ACV OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2acv ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
Crystal structures of a multifunctional triterpene/flavonoid glycosyltransferase from Medicago truncatula., Shao H, He X, Achnine L, Blount JW, Dixon RA, Wang X, Plant Cell. 2005 Nov;17(11):3141-54. Epub 2005 Oct 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16214900 16214900]
</StructureSection>
[[Category: Large Structures]]
[[Category: Medicago truncatula]]
[[Category: Medicago truncatula]]
[[Category: Single protein]]
[[Category: Achnine L]]
[[Category: Achnine, L.]]
[[Category: Blount JW]]
[[Category: Blount, J W.]]
[[Category: Dixon RA]]
[[Category: Dixon, R A.]]
[[Category: He X]]
[[Category: He, X.]]
[[Category: Shao H]]
[[Category: Shao, H.]]
[[Category: Wang X]]
[[Category: Wang, X.]]
[[Category: glycosyltransferase]]
[[Category: udp]]
 
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Retrieved from "https://proteopedia.org/2acv"