1ap7: Difference between revisions

Latest revision as of 08:15, 22 May 2024

P19-INK4D FROM MOUSE, NMR, 20 STRUCTURES

Structural highlights

1ap7 is a 1 chain structure with sequence from Mus musculus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CDN2D_MOUSE Interacts strongly with CDK4 and CDK6 and inhibits them.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

In cancer, the biochemical pathways that are dominated by the two tumour-suppressor proteins, p53 and Rb, are the most frequently disrupted. Cyclin D-dependent kinases phosphorylate Rb to control its activity and they are, in turn, specifically inhibited by the Ink4 family of cyclin-dependent kinase inhibitors (CDKIs) which cause arrest at the G1 phase of the cell cycle. Mutations in Rb, cyclin D1, its catalytic subunit Cdk4, and the CDKI p16Ink4a, which alter the protein or its level of expression, are all strongly implicated in cancer. This suggests that the Rb 'pathway' is of particular importance. Here we report the structure of the p19Ink4d protein, determined by NMR spectroscopy. The structure indicates that most mutations to the p16Ink4a gene, which result in loss of function, are due to incorrectly folded and/or insoluble proteins. We propose a model for the interaction of Ink4 proteins with D-type cyclin-Cdk4/6 complexes that might provide a basis for the design of therapeutics against cancer. The sequences of the Ink4 family of CDKIs are highly conserved

Structure of the cyclin-dependent kinase inhibitor p19Ink4d.,Luh FY, Archer SJ, Domaille PJ, Smith BO, Owen D, Brotherton DH, Raine AR, Xu X, Brizuela L, Brenner SL, Laue ED Nature. 1997 Oct 30;389(6654):999-1003. PMID:9353127^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hirai H, Roussel MF, Kato JY, Ashmun RA, Sherr CJ. Novel INK4 proteins, p19 and p18, are specific inhibitors of the cyclin D-dependent kinases CDK4 and CDK6. Mol Cell Biol. 1995 May;15(5):2672-81. PMID:7739547
↑ Chan FK, Zhang J, Cheng L, Shapiro DN, Winoto A. Identification of human and mouse p19, a novel CDK4 and CDK6 inhibitor with homology to p16ink4. Mol Cell Biol. 1995 May;15(5):2682-8. PMID:7739548
↑ Luh FY, Archer SJ, Domaille PJ, Smith BO, Owen D, Brotherton DH, Raine AR, Xu X, Brizuela L, Brenner SL, Laue ED. Structure of the cyclin-dependent kinase inhibitor p19Ink4d. Nature. 1997 Oct 30;389(6654):999-1003. PMID:9353127 doi:10.1038/40202

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Hirai H, Roussel MF, Kato JY, Ashmun RA, Sherr CJ. Novel INK4 proteins, p19 and p18, are specific inhibitors of the cyclin D-dependent kinases CDK4 and CDK6. Mol Cell Biol. 1995 May;15(5):2672-81. PMID:7739547

[2] Chan FK, Zhang J, Cheng L, Shapiro DN, Winoto A. Identification of human and mouse p19, a novel CDK4 and CDK6 inhibitor with homology to p16ink4. Mol Cell Biol. 1995 May;15(5):2682-8. PMID:7739548

[3] Luh FY, Archer SJ, Domaille PJ, Smith BO, Owen D, Brotherton DH, Raine AR, Xu X, Brizuela L, Brenner SL, Laue ED. Structure of the cyclin-dependent kinase inhibitor p19Ink4d. Nature. 1997 Oct 30;389(6654):999-1003. PMID:9353127 doi:10.1038/40202

[1]

[2]

[3]

@@ Line 1: / Line 1: @@
 ==P19-INK4D FROM MOUSE, NMR, 20 STRUCTURES==
-<StructureSection load='1ap7' size='340' side='right' caption='[[1ap7]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='1ap7' size='340' side='right'caption='[[1ap7]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ap7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AP7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ap7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AP7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AP7 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ap7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ap7 OCA], [http://pdbe.org/1ap7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ap7 RCSB], [http://www.ebi.ac.uk/pdbsum/1ap7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ap7 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ap7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ap7 OCA], [https://pdbe.org/1ap7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ap7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ap7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ap7 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CDN2D_MOUSE CDN2D_MOUSE]] Interacts strongly with CDK4 and CDK6 and inhibits them.<ref>PMID:7739547</ref> <ref>PMID:7739548</ref>
+[https://www.uniprot.org/uniprot/CDN2D_MOUSE CDN2D_MOUSE] Interacts strongly with CDK4 and CDK6 and inhibits them.<ref>PMID:7739547</ref> <ref>PMID:7739548</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
    <jmolCheckbox>
-     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ap/1ap7_consurf.spt"</scriptWhenChecked>
+     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ap/1ap7_consurf.spt"</scriptWhenChecked>
      <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
      <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 31: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Lk3 transgenic mice]]
+[[Category: Large Structures]]
-[[Category: Archer, S J]]
+[[Category: Mus musculus]]
-[[Category: Domaille, P J]]
+[[Category: Archer SJ]]
-[[Category: Laue, E D]]
+[[Category: Domaille PJ]]
-[[Category: Luh, F Y]]
+[[Category: Laue ED]]
-[[Category: Smith, B O]]
+[[Category: Luh FY]]
-[[Category: Ankyrin repeat]]
+[[Category: Smith BO]]
-[[Category: Cdki]]
-[[Category: Cell cycle inhibitor]]
-[[Category: Cyclin dependent kinase inhibitor]]
-[[Category: Ink]]

1ap7: Difference between revisions

Latest revision as of 08:15, 22 May 2024

P19-INK4D FROM MOUSE, NMR, 20 STRUCTURES

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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