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[[Image:2ann.gif|left|200px]]


{{Structure
==Crystal structure (I) of Nova-1 KH1/KH2 domain tandem with 25 nt RNA hairpin==
|PDB= 2ann |SIZE=350|CAPTION= <scene name='initialview01'>2ann</scene>, resolution 2.300&Aring;
<StructureSection load='2ann' size='340' side='right'caption='[[2ann]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
<table><tr><td colspan='2'>[[2ann]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ANN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ANN FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
|GENE= Nova1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ann FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ann OCA], [https://pdbe.org/2ann PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ann RCSB], [https://www.ebi.ac.uk/pdbsum/2ann PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ann ProSAT]</span></td></tr>
|RELATEDENTRY=[[2anr|2ANR]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ann FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ann OCA], [http://www.ebi.ac.uk/pdbsum/2ann PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ann RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/NOVA1_HUMAN NOVA1_HUMAN] May regulate RNA splicing or metabolism in a specific subset of developing neurons.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/an/2ann_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ann ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Nova onconeural antigens are neuron-specific RNA-binding proteins implicated in paraneoplastic opsoclonus-myoclonus-ataxia (POMA) syndrome. Nova harbors three K-homology (KH) motifs implicated in alternate splicing regulation of genes involved in inhibitory synaptic transmission. We report the crystal structure of the first two KH domains (KH1/2) of Nova-1 bound to an in vitro selected RNA hairpin, containing a UCAG-UCAC high-affinity binding site. Sequence-specific intermolecular contacts in the complex involve KH1 and the second UCAC repeat, with the RNA scaffold buttressed by interactions between repeats. Whereas the canonical RNA-binding surface of KH2 in the above complex engages in protein-protein interactions in the crystalline state, the individual KH2 domain can sequence-specifically target the UCAC RNA element in solution. The observed antiparallel alignment of KH1 and KH2 domains in the crystal structure of the complex generates a scaffold that could facilitate target pre-mRNA looping on Nova binding, thereby potentially explaining Nova's functional role in splicing regulation.


'''Crystal structure (I) of Nova-1 KH1/KH2 domain tandem with 25 nt RNA hairpin'''
Protein-RNA and protein-protein recognition by dual KH1/2 domains of the neuronal splicing factor Nova-1.,Teplova M, Malinina L, Darnell JC, Song J, Lu M, Abagyan R, Musunuru K, Teplov A, Burley SK, Darnell RB, Patel DJ Structure. 2011 Jul 13;19(7):930-44. PMID:21742260<ref>PMID:21742260</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==About this Structure==
</div>
2ANN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ANN OCA].
<div class="pdbe-citations 2ann" style="background-color:#fffaf0;"></div>
[[Category: Homo sapiens]]
== References ==
[[Category: Single protein]]
<references/>
[[Category: Burley, S K.]]
__TOC__
[[Category: Darnell, J C.]]
</StructureSection>
[[Category: Darnell, R B.]]
[[Category: Large Structures]]
[[Category: Malinina, L.]]
[[Category: Mus musculus]]
[[Category: Musunuru, K.]]
[[Category: Burley SK]]
[[Category: Patel, D J.]]
[[Category: Darnell JC]]
[[Category: Teplov, A.]]
[[Category: Darnell RB]]
[[Category: Teplova, M.]]
[[Category: Malinina L]]
[[Category: protein-rna complex]]
[[Category: Musunuru K]]
 
[[Category: Patel DJ]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:54:59 2008''
[[Category: Teplov A]]
[[Category: Teplova M]]

Latest revision as of 03:50, 6 June 2025

Crystal structure (I) of Nova-1 KH1/KH2 domain tandem with 25 nt RNA hairpin

2ann, resolution 2.30Å

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