2b5a: Difference between revisions

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[[Image:2b5a.gif|left|200px]]


{{Structure
==C.BclI, Control Element of the BclI Restriction-Modification System==
|PDB= 2b5a |SIZE=350|CAPTION= <scene name='initialview01'>2b5a</scene>, resolution 1.543&Aring;
<StructureSection load='2b5a' size='340' side='right'caption='[[2b5a]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>
<table><tr><td colspan='2'>[[2b5a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_caldolyticus Bacillus caldolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B5A FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.543&#8491;</td></tr>
|GENE= bclIC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1394 Bacillus caldolyticus])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5a OCA], [https://pdbe.org/2b5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b5a RCSB], [https://www.ebi.ac.uk/pdbsum/2b5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b5a ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b5a OCA], [http://www.ebi.ac.uk/pdbsum/2b5a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2b5a RCSB]</span>
== Evolutionary Conservation ==
}}
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
'''C.BclI, Control Element of the BclI Restriction-Modification System'''
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b5/2b5a_consurf.spt"</scriptWhenChecked>
 
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
==Overview==
    <text>to colour the structure by Evolutionary Conservation</text>
Protection from DNA invasion is afforded by restriction-modification systems in many bacteria. The efficiency of protection depends crucially on the relative expression levels of restriction versus methytransferase genes. This regulation is provided by a controller protein, named C protein. Studies of the Bcll system in E. coli suggest that C.Bcll functions as a negative regulator for M.Bcll expression, implying that it plays a role in defense against foreign DNA during virus infection. C.Bcll binds (Kd = 14.3 nM) to a 2-fold symmetric C box DNA sequence that overlaps with the putative -35 promoter region upstream of the bcllM and bcllC genes. The C.Bcll fold comprises five alpha helices: two helices form a helix-turn-helix motif, and the remaining three helices form the extensive dimer interface. The C.Bcll-DNA model proposed suggests that DNA bending might play an important role in gene regulation, and that Glu27 and Asp31 in C.Bcll might function critically in the regulation.
  </jmolCheckbox>
 
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b5a ConSurf].
==About this Structure==
<div style="clear:both"></div>
2B5A is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_caldolyticus Bacillus caldolyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B5A OCA].  
__TOC__
 
</StructureSection>
==Reference==
[[Category: Large Structures]]
Crystal structure of the restriction-modification system control element C.Bcll and mapping of its binding site., Sawaya MR, Zhu Z, Mersha F, Chan SH, Dabur R, Xu SY, Balendiran GK, Structure. 2005 Dec;13(12):1837-47. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16338412 16338412]
[[Category: Balendiran GK]]
[[Category: Bacillus caldolyticus]]
[[Category: Chan SH]]
[[Category: Protein complex]]
[[Category: Dabur R]]
[[Category: Balendiran, G K.]]
[[Category: Mersha F]]
[[Category: Chan, S H.]]
[[Category: Sawaya MR]]
[[Category: Dabur, R.]]
[[Category: Xu SY]]
[[Category: Mersha, F.]]
[[Category: Zhu Z]]
[[Category: Sawaya, M R.]]
[[Category: Xu, S Y.]]
[[Category: Zhu, Z.]]
[[Category: helix-turn-helix motif]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:01:42 2008''
Retrieved from "https://proteopedia.org/2b5a"