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==Structure of Shigella effector LRR domain==
==Structure of Shigella effector LRR domain==
<StructureSection load='5b0n' size='340' side='right' caption='[[5b0n]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='5b0n' size='340' side='right'caption='[[5b0n]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5b0n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B0N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5B0N FirstGlance]. <br>
<table><tr><td colspan='2'>[[5b0n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B0N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B0N FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ipaH9.8, CP0226, pWR501_0234, SFLP090 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5b0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b0n OCA], [http://pdbe.org/5b0n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5b0n RCSB], [http://www.ebi.ac.uk/pdbsum/5b0n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5b0n ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b0n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b0n OCA], [https://pdbe.org/5b0n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b0n RCSB], [https://www.ebi.ac.uk/pdbsum/5b0n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b0n ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/IPA9_SHIFL IPA9_SHIFL]] Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is an E3 ubiquitin ligase that interferes with host's ubiquitination pathway and modulates the acute inflammatory responses, thus facilitating bacterial colonization within the host cell. Interacts with IKBKG (NEMO) and TNIP1 (ABIN-1), an ubiquitin-binding adapter protein, which results in TNIP1-dependent 'Lys-27'-linked polyubiquitination of IKBKG. Consequently, polyubiquitinated IKBKG undergoes proteasome-dependent degradation, which perturbs NF-kappa-B activation during bacterial infection. Uses UBE2D2 (UBCH5B) as an E2 ubiquitin-conjugating enzyme.<ref>PMID:15950937</ref> <ref>PMID:18005683</ref> <ref>PMID:20010814</ref>
[https://www.uniprot.org/uniprot/IPA9_SHIFL IPA9_SHIFL] Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is an E3 ubiquitin ligase that interferes with host's ubiquitination pathway and modulates the acute inflammatory responses, thus facilitating bacterial colonization within the host cell. Interacts with IKBKG (NEMO) and TNIP1 (ABIN-1), an ubiquitin-binding adapter protein, which results in TNIP1-dependent 'Lys-27'-linked polyubiquitination of IKBKG. Consequently, polyubiquitinated IKBKG undergoes proteasome-dependent degradation, which perturbs NF-kappa-B activation during bacterial infection. Uses UBE2D2 (UBCH5B) as an E2 ubiquitin-conjugating enzyme.<ref>PMID:15950937</ref> <ref>PMID:18005683</ref> <ref>PMID:20010814</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Infectious diseases caused by bacteria have significant impacts on global public health. During infection, pathogenic bacteria deliver a variety of virulence factors, called effectors, into host cells. The Shigella effector IpaH9.8 functions as an ubiquitin ligase, ubiquitinating the NF-kappaB essential modulator (NEMO)/IKK-gamma to inhibit host inflammatory responses. IpaH9.8 contains leucine-rich repeats (LRRs) involved in substrate recognition and an E3 ligase domain. To elucidate the structural basis of the function of IpaH9.8, the crystal structure of the LRR domain of Shigella IpaH9.8 was determined and this structure was compared with the known structures of other IpaH family members. This model provides insights into the structural features involved in substrate specificity.


Crystal structure of the substrate-recognition domain of the Shigella E3 ligase IpaH9.8.,Takagi K, Kim M, Sasakawa C, Mizushima T Acta Crystallogr F Struct Biol Commun. 2016 Apr 1;72(Pt 4):269-75. doi:, 10.1107/S2053230X16002715. Epub 2016 Mar 16. PMID:27050259<ref>PMID:27050259</ref>
==See Also==
 
*[[Ubiquitin protein ligase 3D structures|Ubiquitin protein ligase 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5b0n" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Shigella paradysenteriae weldin 1927]]
[[Category: Large Structures]]
[[Category: Kim, M]]
[[Category: Shigella flexneri]]
[[Category: Mizushima, T]]
[[Category: Kim M]]
[[Category: Sasakawa, C]]
[[Category: Mizushima T]]
[[Category: Takagi, K]]
[[Category: Sasakawa C]]
[[Category: Effector]]
[[Category: Takagi K]]
[[Category: Ligase]]
[[Category: Lrr domain]]
[[Category: Ubiquitin ligase]]
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