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| [[Image:2b59.gif|left|200px]]
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| {{Structure
| | ==The type II cohesin dockerin complex== |
| |PDB= 2b59 |SIZE=350|CAPTION= <scene name='initialview01'>2b59</scene>, resolution 2.11Å
| | <StructureSection load='2b59' size='340' side='right'caption='[[2b59]], [[Resolution|resolution]] 2.11Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
| | <table><tr><td colspan='2'>[[2b59]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus Acetivibrio thermocellus] and [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus_ATCC_27405 Acetivibrio thermocellus ATCC 27405]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B59 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B59 FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.11Å</td></tr> |
| |GENE= SdbA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=203119 Clostridium thermocellum ATCC 27405]), cipA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 Clostridium thermocellum])
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| |DOMAIN= | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b59 OCA], [https://pdbe.org/2b59 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b59 RCSB], [https://www.ebi.ac.uk/pdbsum/2b59 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b59 ProSAT], [https://www.topsan.org/Proteins/BSGI/2b59 TOPSAN]</span></td></tr> |
| |RELATEDENTRY=
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b59 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b59 OCA], [http://www.ebi.ac.uk/pdbsum/2b59 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2b59 RCSB]</span>
| | == Function == |
| }}
| | [https://www.uniprot.org/uniprot/A3DF10_ACET2 A3DF10_ACET2] |
| | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> |
| | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b5/2b59_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> |
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b59 ConSurf]. |
| | <div style="clear:both"></div> |
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| '''The type II cohesin dockerin complex'''
| | ==See Also== |
| | | *[[Cellulosome scaffolding protein 3D structures|Cellulosome scaffolding protein 3D structures]] |
| | | __TOC__ |
| ==Overview== | | </StructureSection> |
| Bacterial cell-surface attachment of macromolecular complexes maintains the microorganism in close proximity to extracellular substrates and allows for optimal uptake of hydrolytic byproducts. The cellulosome is a large multienzyme complex used by many anaerobic bacteria for the efficient degradation of plant cell-wall polysaccharides. The mechanism of cellulosome retention to the bacterial cell surface involves a calcium-mediated protein-protein interaction between the dockerin (Doc) module from the cellulosomal scaffold and a cohesin (Coh) module of cell-surface proteins located within the proteoglycan layer. Here, we report the structure of an ultra-high-affinity (K(a) = 1.44 x 10(10) M(-1)) complex between type II Doc, together with its neighboring X module from the cellulosome scaffold of Clostridium thermocellum, and a type II Coh module associated with the bacterial cell surface. Identification of X module-Doc and X module-Coh contacts reveal roles for the X module in Doc stability and enhanced Coh recognition. This extremely tight interaction involves one face of the Coh and both helices of the Doc and comprises significant hydrophobic character and a complementary extensive hydrogen-bond network. This structure represents a unique mechanism for cell-surface attachment in anaerobic bacteria and provides a rationale for discriminating between type I and type II Coh modules.
| | [[Category: Acetivibrio thermocellus]] |
| | | [[Category: Acetivibrio thermocellus ATCC 27405]] |
| ==About this Structure==
| | [[Category: Large Structures]] |
| 2B59 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum] and [http://en.wikipedia.org/wiki/Clostridium_thermocellum_atcc_27405 Clostridium thermocellum atcc 27405]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B59 OCA].
| | [[Category: Adams JJ]] |
| | | [[Category: Smith SP]] |
| ==Reference==
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| Mechanism of bacterial cell-surface attachment revealed by the structure of cellulosomal type II cohesin-dockerin complex., Adams JJ, Pal G, Jia Z, Smith SP, Proc Natl Acad Sci U S A. 2006 Jan 10;103(2):305-10. Epub 2005 Dec 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16384918 16384918]
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| [[Category: Clostridium thermocellum]] | |
| [[Category: Clostridium thermocellum atcc 27405]] | |
| [[Category: Protein complex]] | |
| [[Category: Adams, J J.]] | |
| [[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]]
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| [[Category: Smith, S P.]] | |
| [[Category: bsgi]]
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| [[Category: cellulosome]]
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| [[Category: ef hand]]
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| [[Category: montreal-kingston bacterial structural genomics initiative]]
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| [[Category: protein-protein complex]]
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| [[Category: structural genomic]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:01:43 2008''
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