5aec: Difference between revisions
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==Type II Baeyer-Villiger monooxygenase.The oxygenating constituent of 3,6-diketocamphane monooxygenase from CAM plasmid of Pseudomonas putida in complex with FMN.== | ==Type II Baeyer-Villiger monooxygenase.The oxygenating constituent of 3,6-diketocamphane monooxygenase from CAM plasmid of Pseudomonas putida in complex with FMN.== | ||
<StructureSection load='5aec' size='340' side='right' caption='[[5aec]], [[Resolution|resolution]] 1.93Å' scene=''> | <StructureSection load='5aec' size='340' side='right'caption='[[5aec]], [[Resolution|resolution]] 1.93Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5aec]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5aec]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2wgk 2wgk]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AEC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AEC FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PIN:PIPERAZINE-N,N-BIS(2-ETHANESULFONIC+ACID)'>PIN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PIN:PIPERAZINE-N,N-BIS(2-ETHANESULFONIC+ACID)'>PIN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aec FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aec OCA], [https://pdbe.org/5aec PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aec RCSB], [https://www.ebi.ac.uk/pdbsum/5aec PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aec ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/36DKM_PSEPU 36DKM_PSEPU] Involved in the degradation and assimilation of (-)-camphor, which allows P.putida strain NCIMB 10007 to grow on this enantiomer of camphor as the sole carbon source (PubMed:8515237). Catalyzes the FMNH(2)-dependent lactonization of 3,6-diketocamphane via a Baeyer-Villiger oxidation to produce the unstable lactone 5-oxo-1,2-campholide with (S,S) configuration, that presumably undergoes spontaneous hydrolysis to form 2-oxo-Delta(3)-4,5,5-trimethylcyclopentenylacetate (PubMed:23524667). Is also able to convert (-)-camphor to the corresponding lactone in vitro (PubMed:23524667, PubMed:22286514, PubMed:8515237). Shows no conversion of (+)-camphor, (+)-fenchone, (-)-fenchone, and (+)-nopinone. Acts on other bicyclic ketones but very poorly on a few 2- and 4-substituted monocyclic ketones (PubMed:23524667).<ref>PMID:22286514</ref> <ref>PMID:23524667</ref> <ref>PMID:8515237</ref> <ref>PMID:8515237</ref> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 5aec" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5aec" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Monooxygenase 3D structures|Monooxygenase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Beecher | [[Category: Pseudomonas putida]] | ||
[[Category: Bornscheuer | [[Category: Beecher J]] | ||
[[Category: Bourenkov | [[Category: Bornscheuer UT]] | ||
[[Category: Davenport | [[Category: Bourenkov G]] | ||
[[Category: Dcunha | [[Category: Davenport CF]] | ||
[[Category: Donadio | [[Category: Dcunha S]] | ||
[[Category: Gibson | [[Category: Donadio G]] | ||
[[Category: Hasegawa | [[Category: Gibson RP]] | ||
[[Category: Isupov | [[Category: Hasegawa Y]] | ||
[[Category: Iwaki | [[Category: Isupov MN]] | ||
[[Category: Kadow | [[Category: Iwaki H]] | ||
[[Category: Lau | [[Category: Kadow M]] | ||
[[Category: Littlechild | [[Category: Lau PC]] | ||
[[Category: Loschinski | [[Category: Littlechild JA]] | ||
[[Category: McGhie | [[Category: Loschinski K]] | ||
[[Category: Saneei | [[Category: McGhie EJ]] | ||
[[Category: Sayer | [[Category: Saneei V]] | ||
[[Category: Schroeder | [[Category: Sayer C]] | ||
[[Category: Schroeder E]] | |||
