4o87: Difference between revisions

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==Crystal structure of a N-tagged Nuclease==
==Crystal structure of a N-tagged Nuclease==
<StructureSection load='4o87' size='340' side='right' caption='[[4o87]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='4o87' size='340' side='right'caption='[[4o87]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4o87]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_22303 Atcc 22303]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O87 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O87 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4o87]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Millerozyma_acaciae Millerozyma acaciae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O87 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O87 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4o88|4o88]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o87 OCA], [http://pdbe.org/4o87 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o87 RCSB], [http://www.ebi.ac.uk/pdbsum/4o87 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o87 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o87 OCA], [https://pdbe.org/4o87 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o87 RCSB], [https://www.ebi.ac.uk/pdbsum/4o87 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o87 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q707V3_9ASCO Q707V3_9ASCO]
tRNA restriction by anticodon nucleases underlies cellular stress responses and self-nonself discrimination in a wide range of taxa. Anticodon breakage inhibits protein synthesis, which, in turn, results in growth arrest or cell death. The eukaryal ribotoxin PaT secreted by Pichia acaciae inhibits growth of Saccharomyces cerevisiae via cleavage of tRNA(Gln(UUG)). We find that recombinant PaT incises a synthetic tRNA(Gln(UUG)) stem-loop RNA by transesterification at a single site 3' of the wobble uridine, yielding 2',3'-cyclic phosphate and 5'-OH ends. Incision is suppressed by replacement of the wobble nucleobase with adenine or guanine. The crystal structure of PaT reveals a distinctive fold and active site, essential components of which are demonstrated by mutagenesis. Pichia acaciae evades self-toxicity via a distinctive intracellular immunity protein, ImmPaT, which binds PaT and blocks nuclease activity. Our results highlight the evolutionary diversity of tRNA restriction and immunity systems.
 
Structure, mechanism, and specificity of a eukaryal tRNA restriction enzyme involved in self-nonself discrimination.,Chakravarty AK, Smith P, Jalan R, Shuman S Cell Rep. 2014 Apr 24;7(2):339-47. doi: 10.1016/j.celrep.2014.03.034. Epub 2014, Apr 13. PMID:24726365<ref>PMID:24726365</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4o87" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 22303]]
[[Category: Large Structures]]
[[Category: Chakravarty, A K]]
[[Category: Millerozyma acaciae]]
[[Category: Shuman, S]]
[[Category: Chakravarty AK]]
[[Category: Smith, P]]
[[Category: Shuman S]]
[[Category: Hydrolase]]
[[Category: Smith P]]
[[Category: Novel fold]]
[[Category: Nuclease]]

Latest revision as of 12:38, 1 March 2024

Crystal structure of a N-tagged Nuclease

4o87, resolution 1.80Å

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