4qxa: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:


==Crystal structure of the Rab9A-RUTBC2 RBD complex==
==Crystal structure of the Rab9A-RUTBC2 RBD complex==
<StructureSection load='4qxa' size='340' side='right' caption='[[4qxa]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='4qxa' size='340' side='right'caption='[[4qxa]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4qxa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QXA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4QXA FirstGlance]. <br>
<table><tr><td colspan='2'>[[4qxa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QXA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QXA FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rab9a, Rab9, Sid99 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Sgsm1, Rutbc2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4qxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qxa OCA], [http://pdbe.org/4qxa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4qxa RCSB], [http://www.ebi.ac.uk/pdbsum/4qxa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4qxa ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qxa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qxa OCA], [https://pdbe.org/4qxa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qxa RCSB], [https://www.ebi.ac.uk/pdbsum/4qxa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qxa ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/RAB9A_MOUSE RAB9A_MOUSE]] Involved in the transport of proteins between the endosomes and the trans-Golgi network (By similarity).  
[https://www.uniprot.org/uniprot/RAB9A_MOUSE RAB9A_MOUSE] Involved in the transport of proteins between the endosomes and the trans-Golgi network (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Rab9 plays a vital role in regulating the transport of mannose 6-phosphate receptors from late endosomes to the trans-Golgi network through interactions with various effectors. Here, we report the crystal structure of GTP-bound Rab9A in complex with the Rab-binding domain (RBD) of the effector RUTBC2. RUTBC2 RBD assumes a pleckstrin homology domain fold that uses a binding site consisting of mainly beta1 and the eta1 insertion to interact with the switch and interswitch regions of Rab9A. The C-terminal hypervariable region of Rab9A is disordered and thus not required for RUTBC2 binding. The conformational plasticity of the switch and interswitch regions of Rab9A primarily determines the specificity for RUTBC2. Our biochemical and biological data confirm these findings and further show that Rab9B can bind to RUTBC2 probably in a similar manner as Rab9A. These results together reveal the molecular basis for the binding specificity of Rab9A with RUTBC2.
 
Crystal structure of the Rab9A-RUTBC2 RBD complex reveals the molecular basis for the binding specificity of Rab9A with RUTBC2.,Zhang Z, Wang S, Shen T, Chen J, Ding J Structure. 2014 Oct 7;22(10):1408-20. doi: 10.1016/j.str.2014.08.005. Epub 2014, Sep 11. PMID:25220469<ref>PMID:25220469</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4qxa" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Ras-related protein Rab 3D structures|Ras-related protein Rab 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Lk3 transgenic mice]]
[[Category: Large Structures]]
[[Category: Ding, J]]
[[Category: Mus musculus]]
[[Category: Wang, S]]
[[Category: Ding J]]
[[Category: Zhang, Z]]
[[Category: Wang S]]
[[Category: Ph domain]]
[[Category: Zhang Z]]
[[Category: Protein transport-protein binding complex]]
[[Category: Rab binding domain]]
[[Category: Rab9-effector complex]]
[[Category: Rab9a]]
[[Category: Rutbc2]]
Retrieved from "https://proteopedia.org/4qxa"