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| ==Structure of hypothetical Staphylococcus protein SA0856 with zinc== | | ==Structure of hypothetical Staphylococcus protein SA0856 with zinc== |
| <StructureSection load='5kef' size='340' side='right' caption='[[5kef]], [[Resolution|resolution]] 2.23Å' scene=''> | | <StructureSection load='5kef' size='340' side='right'caption='[[5kef]], [[Resolution|resolution]] 2.23Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[5kef]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_cn1 Staphylococcus aureus subsp. aureus cn1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KEF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5KEF FirstGlance]. <br> | | <table><tr><td colspan='2'>[[5kef]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_CN1 Staphylococcus aureus subsp. aureus CN1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5KEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5KEF FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.23Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3l20|3l20]]</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SAKOR_02586 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1193576 Staphylococcus aureus subsp. aureus CN1])</td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5kef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kef OCA], [https://pdbe.org/5kef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5kef RCSB], [https://www.ebi.ac.uk/pdbsum/5kef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5kef ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5kef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5kef OCA], [http://pdbe.org/5kef PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5kef RCSB], [http://www.ebi.ac.uk/pdbsum/5kef PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5kef ProSAT]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/T1YBT4_STAAU T1YBT4_STAAU] |
| The crystal structures of protein SA0856 from Staphylococcus aureus in its apo-form and in complex with a Zn2+-ion have been presented. The 152 amino acid protein consists of two similar domains with alpha + beta topology. In both crystalline state and in solution, the protein forms a dimer with monomers related by a twofold pseudo-symmetry rotation axis. A sequence homology search identified the protein as a member of the structural family Glyoxalase I. We have shown that the enzyme possesses glyoxalase I activity in the presence of Zn2+, Mg2+, Ni2+, and Co2+, in this order of preference. Sequence and structure comparisons revealed that human glyoxalase I should be assigned to a subfamily A, while S. aureus glyoxalase I represents a new subfamily B, which includes also proteins from other bacteria. Both subfamilies have a similar protein chain fold but rather diverse sequences. The active sites of human and staphylococcus glyoxalases I are also different: the former contains one Zn-ion per chain; the latter incorporates two of these ions. In the active site of SA0856, the first Zn-ion is well coordinated by His58, Glu60 from basic molecule and Glu40*, His44* from adjacent symmetry-related molecule. The second Zn3-ion is coordinated only by residue His143 from protein molecule and one acetate ion. We suggest that only single Zn1-ion plays the role of catalytic center. The newly found differences between the two subfamilies could guide the design of new drugs against S. aureus, an important pathogenic micro-organism.
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| Crystal structure of Staphylococcus aureus Zn-glyoxalase I: new subfamily of glyoxalase I family.,Chirgadze YN, Boshkova EA, Battaile KP, Mendes VG, Lam R, Chan TS, Romanov V, Pai EF, Chirgadze NY J Biomol Struct Dyn. 2017 Jan 16:1-11. doi: 10.1080/07391102.2016.1278038. PMID:28034013<ref>PMID:28034013</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 5kef" style="background-color:#fffaf0;"></div>
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| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Staphylococcus aureus subsp. aureus cn1]] | | [[Category: Large Structures]] |
| [[Category: Battaile, K P]] | | [[Category: Staphylococcus aureus subsp. aureus CN1]] |
| [[Category: Chan, T]] | | [[Category: Battaile KP]] |
| [[Category: Chirgadze, N Y]] | | [[Category: Chan T]] |
| [[Category: Chirgadze, Y N]] | | [[Category: Chirgadze NY]] |
| [[Category: Lam, R]] | | [[Category: Chirgadze YN]] |
| [[Category: Mendez, V]] | | [[Category: Lam R]] |
| [[Category: Mihajlovic, V]] | | [[Category: Mendez V]] |
| [[Category: Pai, E]] | | [[Category: Mihajlovic V]] |
| [[Category: Romanov, V]] | | [[Category: Pai E]] |
| [[Category: Glyoxalase]]
| | [[Category: Romanov V]] |
| [[Category: Metal binding protein]]
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| [[Category: Metalloprotein]]
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