6ao7: Difference between revisions
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==Crystal Structure of a GNAT family acetyltransferase from Elizabethkingia anophelis with acetyl-CoA bound== | ==Crystal Structure of a GNAT family acetyltransferase from Elizabethkingia anophelis with acetyl-CoA bound== | ||
<StructureSection load='6ao7' size='340' side='right' caption='[[6ao7]], [[Resolution|resolution]] 1.85Å' scene=''> | <StructureSection load='6ao7' size='340' side='right'caption='[[6ao7]], [[Resolution|resolution]] 1.85Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6ao7]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[6ao7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Elizabethkingia_anophelis Elizabethkingia anophelis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AO7 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ao7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ao7 OCA], [https://pdbe.org/6ao7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ao7 RCSB], [https://www.ebi.ac.uk/pdbsum/6ao7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ao7 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
General control non-repressible 5 (GCN5)-related N-acetyltransferases (GNATs) catalyse the acetylation of a diverse range of substrates, thereby orchestrating a variety of biological processes within prokaryotes and eukaryotes. GNAT enzymes can catalyze the transfer of an acetyl group from acetyl coenzyme A to substrates such as aminoglycoside antibiotics, amino acids, polyamines, peptides, vitamins, catecholamines, and large macromolecules including proteins. Although GNATs generally exhibit low to moderate sequence identity, they share a conserved catalytic fold and conserved structural motifs. In this current study we characterize the high-resolution X-ray crystallographic structure of a GNAT enzyme bound with acetyl-CoA from Elizabethkingia anophelis, an important multi-drug resistant bacterium. The tertiary structure is comprised of six alpha-helices and nine beta-strands, and is similar with other GNATs. We identify a new and uncharacterized GNAT dimer interface, which is conserved in at least two other unpublished GNAT structures. This suggests that GNAT enzymes can form at least five different types of dimers, in addition to a range of other oligomers including trimer, tetramer, hexamer, and dodecamer assemblies. The high-resolution structure presented in this study is suitable for future in-silico docking and structure-activity relationship studies. | |||
Structural characterization of a GNAT family acetyltransferase from Elizabethkingia anophelis bound to acetyl-CoA reveals a new dimeric interface.,Shirmast P, Ghafoori SM, Irwin RM, Abendroth J, Mayclin SJ, Lorimer DD, Edwards TE, Forwood JK Sci Rep. 2021 Jan 14;11(1):1274. doi: 10.1038/s41598-020-79649-5. PMID:33446675<ref>PMID:33446675</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6ao7" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Elizabethkingia anophelis]] | ||
[[Category: | [[Category: Large Structures]] | ||
Latest revision as of 14:21, 4 October 2023
Crystal Structure of a GNAT family acetyltransferase from Elizabethkingia anophelis with acetyl-CoA bound
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