IspG: Difference between revisions
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Michal Harel (talk | contribs) New page: <StructureSection load='4g9p' size='340' side='right' caption='IspG containing 4Fe4S cluster complex with MEcPP (PDB entry 4g9p)' scene=''> == Function == IspG is a 4Fe4S protein w... |
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<StructureSection load='4g9p' size='400' side='right' caption='IspG containing 4Fe4S cluster complex with MEcPP, MES, K+ (purple) and Cl- (green) ions (PDB entry [[4g9p]])' scene='77/774679/Cv/1'> | |||
<StructureSection load='4g9p' size=' | |||
== Function == | == Function == | ||
IspG is a 4Fe4S protein which serves as the penultimate enzyme of the non-mevalonate pathway for the biosynthesis os isoprenoid precursors like isopentenyl diphosphate and dimethylallyl <ref>PMID:20932974</ref>. It catalyzes the ring opening of 2C-methyl-D-erythritol 2,4-cyclodiphosphate (MEcPP) producing (E)-1-hydroxyl-2-methyl-but-2-enyl-4-diphosphate (HMBPP). | '''IspG''' or '''4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase''' is a 4Fe4S protein which serves as the penultimate enzyme of the non-mevalonate pathway for the biosynthesis os isoprenoid precursors like isopentenyl diphosphate and dimethylallyl <ref>PMID:20932974</ref>. It catalyzes the ring opening of 2C-methyl-D-erythritol 2,4-cyclodiphosphate (MEcPP) producing (E)-1-hydroxyl-2-methyl-but-2-enyl-4-diphosphate (HMBPP). | ||
== Relevance == | == Relevance == | ||
The non- | The non-mevalonate enzymes are a target for anti-tuberculosis and antimalarial drugs<ref>PMID:22586085</ref>. | ||
== Structural highlights == | == Structural highlights == | ||
The substrate <scene name='77/774679/Cv/2'>MEcPP is located in the cavity between the TIM-barrel and the lid domain</scene> of IpsG. <scene name='77/774679/Cv/3'>It binds covalently to the 4Fe4S cluster</scene> and its <scene name='77/774679/Cv/8'>PP moiety binds to 4 Arg and 1 Lys sidechains</scene>. | |||
*<scene name='77/774679/Cv/6'>4Fe4S cluster binding site</scene>. | |||
*<scene name='77/774679/Cv/7'>Whole active site</scene>. | |||
*<scene name='77/774679/Cv/9'>1st Cl coordination site</scene>. | |||
*<scene name='77/774679/Cv/10'>Cl/K cluster coordination site</scene>. | |||
</StructureSection> | </StructureSection> | ||
== 3D Structures of IspG == | == 3D Structures of IspG == | ||
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[[4s39]], [[4s23]] – TtIspG + HMBPP <br /> | [[4s39]], [[4s23]] – TtIspG + HMBPP <br /> | ||
[[4s3a]], [[4s3b]], [[4s3c]] – TtIspG + intermediate <br /> | [[4s3a]], [[4s3b]], [[4s3c]] – TtIspG + intermediate <br /> | ||
[[ | [[4s3d]] – TtIspG + PPi <br /> | ||
[[4s3e]], [[4s3f]] – TtIspG + inhibitor <br /> | [[4s3e]], [[4s3f]] – TtIspG + inhibitor <br /> | ||
Latest revision as of 09:15, 12 June 2023
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3D Structures of IspG
Updated on 12-June-2023
3noy – IspG – Aquifex aeolicus
4mwa – IspG – Bacillus anthracis
2y0f – TtIspG – Thermus thermophilus
4s38, 4g9p – TtIspG + MEcPP
4s39, 4s23 – TtIspG + HMBPP
4s3a, 4s3b, 4s3c – TtIspG + intermediate
4s3d – TtIspG + PPi
4s3e, 4s3f – TtIspG + inhibitor
