6br8: Difference between revisions
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New page: '''Unreleased structure''' The entry 6br8 is ON HOLD Authors: Deng, J., Peng, S., Pathak, P. Description: Structure of A6 reveals a novel lipid transporter [[Category: Unreleased Struc... |
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==Structure of A6 reveals a novel lipid transporter== | |||
<StructureSection load='6br8' size='340' side='right'caption='[[6br8]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6br8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Fowlpox_virus_strain_NVSL Fowlpox virus strain NVSL]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BR8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6OU:[(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl]+(~{Z})-octadec-9-enoate'>6OU</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PGV:(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL+(11E)-OCTADEC-11-ENOATE'>PGV</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6br8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6br8 OCA], [https://pdbe.org/6br8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6br8 RCSB], [https://www.ebi.ac.uk/pdbsum/6br8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6br8 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A6_FOWPN A6_FOWPN] Plays an essential role in immature virion (IV) to mature virion (MV) transition. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cellular membranes are maintained as closed compartments, broken up only transiently during membrane reorganization or lipid transportation. However, open-ended membranes, likely derived from scissions of the endoplasmic reticulum, persist in vaccinia virus-infected cells during the assembly of the viral envelope. A group of viral membrane assembly proteins (VMAPs) were identified as essential for this process. To understand the mechanism of VMAPs, we determined the 2.2-A crystal structure of the largest member, named A6, which is a soluble protein with two distinct domains. The structure of A6 displays a novel protein fold composed mainly of alpha helices. The larger C-terminal domain forms a unique cage that encloses multiple glycerophospholipids with a lipid bilayer-like configuration. The smaller N-terminal domain does not bind lipid but negatively affects lipid binding by A6. Mutations of key hydrophobic residues lining the lipid-binding cage disrupt lipid binding and abolish viral replication. Our results reveal a protein modality for enclosing the lipid bilayer and provide molecular insight into a viral machinery involved in generating and/or stabilizing open-ended membranes. | |||
Structure of a lipid-bound viral membrane assembly protein reveals a modality for enclosing the lipid bilayer.,Pathak PK, Peng S, Meng X, Han Y, Zhang B, Zhang F, Xiang Y, Deng J Proc Natl Acad Sci U S A. 2018 Jun 18. pii: 1805855115. doi:, 10.1073/pnas.1805855115. PMID:29915071<ref>PMID:29915071</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6br8" style="background-color:#fffaf0;"></div> | ||
[[Category: Deng | == References == | ||
[[Category: Peng | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Fowlpox virus strain NVSL]] | |||
[[Category: Large Structures]] | |||
[[Category: Deng J]] | |||
[[Category: Pathak P]] | |||
[[Category: Peng S]] | |||
Latest revision as of 05:06, 21 November 2024
Structure of A6 reveals a novel lipid transporter
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