6f5p: Difference between revisions
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New page: '''Unreleased structure''' The entry 6f5p is ON HOLD Authors: Serna Martin, I., Grimes, J.M. Description: A mechanism for the activation of the influenza virus transcriptase [[Category... |
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==A mechanism for the activation of the influenza virus transcriptase== | |||
<StructureSection load='6f5p' size='340' side='right'caption='[[6f5p]], [[Resolution|resolution]] 4.14Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6f5p]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Fopius_arisanus Fopius arisanus], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Influenza_C_virus_(C/Johannesburg/1/66) Influenza C virus (C/Johannesburg/1/66)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F5P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6F5P FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.14Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6f5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f5p OCA], [https://pdbe.org/6f5p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6f5p RCSB], [https://www.ebi.ac.uk/pdbsum/6f5p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6f5p ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Influenza virus RNA polymerase (FluPol), a heterotrimer composed of PB1, PB2, and PA subunits (P3 in influenza C), performs both transcription and replication of the viral RNA genome. For transcription, FluPol interacts with the C-terminal domain (CTD) of RNA polymerase II (Pol II), which enables FluPol to snatch capped RNA primers from nascent host RNAs. Here, we describe the co-crystal structure of influenza C virus polymerase (FluPolC) bound to a Ser5-phosphorylated CTD (pS5-CTD) peptide. The position of the CTD-binding site at the interface of PB1, P3, and the flexible PB2 C-terminal domains suggests that CTD binding stabilizes the transcription-competent conformation of FluPol. In agreement, both cap snatching and capped primer-dependent transcription initiation by FluPolC are enhanced in the presence of pS5-CTD. Mutations of amino acids in the CTD-binding site reduce viral mRNA synthesis. We propose a model for the activation of the influenza virus transcriptase through its association with pS5-CTD of Pol II. | |||
A Mechanism for the Activation of the Influenza Virus Transcriptase.,Serna Martin I, Hengrung N, Renner M, Sharps J, Martinez-Alonso M, Masiulis S, Grimes JM, Fodor E Mol Cell. 2018 Jun 21;70(6):1101-1110.e4. doi: 10.1016/j.molcel.2018.05.011. Epub, 2018 Jun 14. PMID:29910112<ref>PMID:29910112</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Grimes | <div class="pdbe-citations 6f5p" style="background-color:#fffaf0;"></div> | ||
[[Category: Serna Martin | |||
==See Also== | |||
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Fopius arisanus]] | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Grimes JM]] | |||
[[Category: Serna Martin I]] | |||
