6f7v: Difference between revisions
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New page: '''Unreleased structure''' The entry 6f7v is ON HOLD Authors: Dorival, J., Risser, F., Jacob, C., Collin, S., Drager, G., Kirschning, A., Paris, C., Chagot, B., Gruez, A., Weissman, K.J... |
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==Crystal structure of LkcE E64Q mutant in complex with LC-KA05== | |||
<StructureSection load='6f7v' size='340' side='right'caption='[[6f7v]], [[Resolution|resolution]] 3.03Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6f7v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_rochei_subsp._volubilis Streptomyces rochei subsp. volubilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6F7V FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.03Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CWH:[(2~{S},5~{R},8~{S},11~{S})-1-[(2~{R},3~{R},5~{R},6~{S})-3,5-dimethyl-6-oxidanyl-4-oxidanylidene-oxan-2-yl]-5,11-dimeth+yl-8-oxidanyl-13-[[(2~{S})-2-oxidanylpropanoyl]amino]tridecan-2-yl]+ethanoate'>CWH</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6f7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f7v OCA], [https://pdbe.org/6f7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6f7v RCSB], [https://www.ebi.ac.uk/pdbsum/6f7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6f7v ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/G4V2H3_STRRO G4V2H3_STRRO] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Acquisition of new catalytic activity is a relatively rare evolutionary event. A striking example appears in the pathway to the antibiotic lankacidin, as a monoamine oxidase (MAO) family member, LkcE, catalyzes both an unusual amide oxidation, and a subsequent intramolecular Mannich reaction to form the polyketide macrocycle. We report evidence here for the molecular basis for this dual activity. The reaction sequence involves several essential active site residues and a conformational change likely comprising an interdomain hinge movement. These features, which have not previously been described in the MAO family, both depend on a unique dimerization mode relative to all structurally characterized members. Taken together, these data add weight to the idea that designing new multifunctional enzymes may require changes in both architecture and catalytic machinery. Encouragingly, however, our data also show LkcE to bind alternative substrates, supporting its potential utility as a general cyclization catalyst in synthetic biology. | |||
Insights into a dual function amide oxidase/macrocyclase from lankacidin biosynthesis.,Dorival J, Risser F, Jacob C, Collin S, Drager G, Paris C, Chagot B, Kirschning A, Gruez A, Weissman KJ Nat Commun. 2018 Sep 28;9(1):3998. doi: 10.1038/s41467-018-06323-w. PMID:30266997<ref>PMID:30266997</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6f7v" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: Collin | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Streptomyces rochei subsp. volubilis]] | ||
[[Category: | [[Category: Chagot B]] | ||
[[Category: Paris | [[Category: Collin S]] | ||
[[Category: | [[Category: Dorival J]] | ||
[[Category: Drager G]] | |||
[[Category: Gruez A]] | |||
[[Category: Jacob C]] | |||
[[Category: Kirschning A]] | |||
[[Category: Paris C]] | |||
[[Category: Risser F]] | |||
[[Category: Weissman KJ]] | |||
Latest revision as of 12:26, 9 May 2024
Crystal structure of LkcE E64Q mutant in complex with LC-KA05
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