6f8s: Difference between revisions
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New page: '''Unreleased structure''' The entry 6f8s is ON HOLD Authors: Talavera, A., Loris, R. Description: Toxin-Antitoxin complex GraTA Category: Unreleased Structures [[Category: Loris, ... |
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==Toxin-Antitoxin complex GraTA== | |||
<StructureSection load='6f8s' size='340' side='right'caption='[[6f8s]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6f8s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida] and [https://en.wikipedia.org/wiki/Pseudomonas_putida_KT2440 Pseudomonas putida KT2440]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6F8S FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6f8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f8s OCA], [https://pdbe.org/6f8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6f8s RCSB], [https://www.ebi.ac.uk/pdbsum/6f8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6f8s ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q88MI6_PSEPK Q88MI6_PSEPK] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bacterial toxin-antitoxin (TA) modules are tightly regulated to maintain growth in favorable conditions or growth arrest during stress. A typical regulatory strategy involves the antitoxin binding and repressing its own promoter while the toxin often acts as a co-repressor. Here we show that Pseudomonas putida graTA-encoded antitoxin GraA and toxin GraT differ from other TA proteins in the sense that not the antitoxin but the toxin possesses a flexible region. GraA auto-represses the graTA promoter: two GraA dimers bind cooperatively at opposite sides of the operator sequence. Contrary to other TA modules, GraT is a de-repressor of the graTA promoter as its N-terminal disordered segment prevents the binding of the GraT2A2 complex to the operator. Removal of this region restores operator binding and abrogates Gr aT toxicity. GraTA represents a TA module where a flexible region in the toxin rather than in the antitoxin controls operon expression and toxin activity. | |||
A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.,Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadzi S, Sobott F, Garcia-Pino A, Horak R, Loris R Nat Commun. 2019 Feb 27;10(1):972. doi: 10.1038/s41467-019-08865-z. PMID:30814507<ref>PMID:30814507</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Loris | <div class="pdbe-citations 6f8s" style="background-color:#fffaf0;"></div> | ||
[[Category: Talavera | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pseudomonas putida]] | |||
[[Category: Pseudomonas putida KT2440]] | |||
[[Category: Loris R]] | |||
[[Category: Talavera A]] | |||
