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[[Image:2co1.gif|left|200px]]


{{Structure
==Salmonella enterica SafA pilin in complex with a 19-residue SafA Nte peptide (F17A mutant)==
|PDB= 2co1 |SIZE=350|CAPTION= <scene name='initialview01'>2co1</scene>, resolution 2.40&Aring;
<StructureSection load='2co1' size='340' side='right'caption='[[2co1]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[2co1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CO1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CO1 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2co1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2co1 OCA], [https://pdbe.org/2co1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2co1 RCSB], [https://www.ebi.ac.uk/pdbsum/2co1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2co1 ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=
== Function ==
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2co1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2co1 OCA], [http://www.ebi.ac.uk/pdbsum/2co1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2co1 RCSB]</span>
[https://www.uniprot.org/uniprot/Q8ZRK4_SALTY Q8ZRK4_SALTY]
}}


'''SALMONELLA ENTERICA SAFA PILIN IN COMPLEX WITH A 19-RESIDUE SAFA NTE PEPTIDE (F17A MUTANT)'''
==See Also==
 
*[[Pilin 3D structures|Pilin 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Gram-negative pathogens commonly use the chaperone-usher pathway to assemble adhesive multisubunit fibers on their surface. In the periplasm, subunits are stabilized by a chaperone that donates a beta strand to complement the subunits' truncated immunoglobulin-like fold. Pilus assembly proceeds through a "donor-strand exchange" (DSE) mechanism whereby this complementary beta strand is replaced by the N-terminal extension (Nte) of an incoming pilus subunit. Using X-ray crystallography and real-time electrospray ionization mass spectrometry (ESI-MS), we demonstrate that DSE requires the formation of a transient ternary complex between the chaperone-subunit complex and the Nte of the next subunit to be assembled. The process is crucially dependent on an initiation site (the P5 pocket) needed to recruit the incoming Nte. The data also suggest a capping reaction displacing DSE toward product formation. These results support a zip-in-zip-out mechanism for DSE and a catalytic role for the usher, the molecular platform at which pili are assembled.
[[Category: Large Structures]]
 
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
==About this Structure==
[[Category: Ashcroft AE]]
2CO1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CO1 OCA].
[[Category: Hannan TJ]]
 
[[Category: Hultgren SJ]]
==Reference==
[[Category: Radford SE]]
Donor-strand exchange in chaperone-assisted pilus assembly proceeds through a concerted beta strand displacement mechanism., Remaut H, Rose RJ, Hannan TJ, Hultgren SJ, Radford SE, Ashcroft AE, Waksman G, Mol Cell. 2006 Jun 23;22(6):831-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16793551 16793551]
[[Category: Remaut H]]
[[Category: Protein complex]]
[[Category: Rose RJ]]
[[Category: Salmonella typhimurium]]
[[Category: Waksman G]]
[[Category: Ashcroft, A E.]]
[[Category: Hannan, T J.]]
[[Category: Hultgren, S J.]]
[[Category: Radford, S E.]]
[[Category: Remaut, H.]]
[[Category: Rose, R J.]]
[[Category: Waksman, G.]]
[[Category: fibril protein]]
[[Category: fold complementation]]
[[Category: pilus subunit]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:24:14 2008''

Latest revision as of 06:39, 1 May 2024

Salmonella enterica SafA pilin in complex with a 19-residue SafA Nte peptide (F17A mutant)

2co1, resolution 2.40Å

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