5z25: Difference between revisions

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New page: '''Unreleased structure''' The entry 5z25 is ON HOLD Authors: Oda, A., Nagao, S., Yamanaka, M., Ueda, I., Shibata, N., Higuchi, Y., Hirota, S. Description: Trimeric Alpha-Helix-Inserte...
 
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'''Unreleased structure'''


The entry 5z25 is ON HOLD
==Trimeric Alpha-Helix-Inserted Circular Permutant of Cytochrome c555==
<StructureSection load='5z25' size='340' side='right'caption='[[5z25]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5z25]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z25 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z25 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z25 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z25 OCA], [https://pdbe.org/5z25 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z25 RCSB], [https://www.ebi.ac.uk/pdbsum/5z25 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z25 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O67504_AQUAE O67504_AQUAE]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Highly-ordered protein structures have gained interest for future uses for biomaterials. Herein, we constructed a building block protein (BBP) by the circular permutation of the hyperthermostable Aquifex aeolicus cytochrome (cyt) c555, and assembled BBP into a triangle-shaped trimer and a tetrahedral structure. The angle of the intermolecular interactions of BBP was controlled by cleaving the domain-swapping hinge loop of cyt c555 and connecting the original N- and C-terminal alpha-helices with an alpha-helical linker. We obtained BBP oligomers up to ~40 mers, with a relatively large amount of trimers. According to the X-ray crystallographic analysis of the BBP trimer, the N-terminal region of one BBP molecule interacted intermolecularly with the C-terminal region of another BBP molecule, resulting in a triangle-shaped structure with an edge length of 68 A. Additionally, four trimers assembled into a unique tetrahedral structure in the crystal. These results demonstrate that the circular permutation connecting the original N- and C-terminal alpha-helices with an alpha-helical linker may be useful for constructing organized protein structures.


Authors: Oda, A., Nagao, S., Yamanaka, M., Ueda, I., Shibata, N., Higuchi, Y., Hirota, S.
Construction of a Triangle-Shaped Trimer and a Tetrahedral Structure Using an alpha-Helix-Inserted Circular Permutant of Cytochrome c(5)(5)(5).,Oda A, Nagao S, Yamanaka M, Ueda I, Watanabe H, Uchihashi T, Shibata N, Higuchi Y, Hirota S Chem Asian J. 2018 Feb 26. doi: 10.1002/asia.201800252. PMID:29484831<ref>PMID:29484831</ref>


Description: Trimeric Alpha-Helix-Inserted Circular Permutant of Cytochrome c555
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Nagao, S]]
<div class="pdbe-citations 5z25" style="background-color:#fffaf0;"></div>
[[Category: Hirota, S]]
== References ==
[[Category: Yamanaka, M]]
<references/>
[[Category: Shibata, N]]
__TOC__
[[Category: Ueda, I]]
</StructureSection>
[[Category: Higuchi, Y]]
[[Category: Aquifex aeolicus VF5]]
[[Category: Oda, A]]
[[Category: Large Structures]]
[[Category: Higuchi Y]]
[[Category: Hirota S]]
[[Category: Nagao S]]
[[Category: Oda A]]
[[Category: Shibata N]]
[[Category: Ueda I]]
[[Category: Yamanaka M]]
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