6f9w: Difference between revisions

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'''Unreleased structure'''


The entry 6f9w is ON HOLD  until Paper Publication
==Crystal structure of the LSM domain of LSM14 in complex with a C-terminal peptide of 4E-T==
<StructureSection load='6f9w' size='340' side='right'caption='[[6f9w]], [[Resolution|resolution]] 2.62&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6f9w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F9W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6F9W FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.623&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6f9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f9w OCA], [https://pdbe.org/6f9w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6f9w RCSB], [https://www.ebi.ac.uk/pdbsum/6f9w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6f9w ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LS14A_HUMAN LS14A_HUMAN] Essential for formation of P-bodies, cytoplasmic structures that provide storage sites for non-translating mRNAs.<ref>PMID:16484376</ref> <ref>PMID:17074753</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The LSM domain-containing protein LSM14/Rap55 plays a role in mRNA decapping, translational repression, and RNA granule (P-body) assembly. How LSM14 interacts with the mRNA silencing machinery, including the eIF4E-binding protein 4E-T and the DEAD-box helicase DDX6, is poorly understood. Here we report the crystal structure of the LSM domain of LSM14 bound to a highly conserved C-terminal fragment of 4E-T. The 4E-T C-terminus forms a bi-partite motif that wraps around the N-terminal LSM domain of LSM14. We also determined the crystal structure of LSM14 bound to the C-terminal RecA-like domain of DDX6. LSM14 binds DDX6 via a unique non-contiguous motif with distinct directionality as compared to other DDX6-interacting proteins. Together with mutational and proteomic studies, the LSM14-DDX6 structure reveals that LSM14 has adopted a divergent mode of binding DDX6 in order to support the formation of mRNA silencing complexes and P-body assembly.


Authors: Brandmann, T., Jinek, M.
Molecular architecture of LSM14 interactions involved in the assembly of mRNA silencing complexes.,Brandmann T, Fakim H, Padamsi Z, Youn JY, Gingras AC, Fabian MR, Jinek M EMBO J. 2018 Mar 6. pii: embj.201797869. doi: 10.15252/embj.201797869. PMID:29510985<ref>PMID:29510985</ref>


Description: Crystal structure of the LSM domain of LSM14 in complex with a C-terminal peptide of 4E-T
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Jinek, M]]
<div class="pdbe-citations 6f9w" style="background-color:#fffaf0;"></div>
[[Category: Brandmann, T]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Brandmann T]]
[[Category: Jinek M]]
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